Each PCR reaction contained 10 [micro]l DNA, 17 [micro]l GPR PCR Master 1.0, 1 [micro]l ASR target-specific primer, 1 [micro]l ASR target-specific probe, and 1 [micro]l water, except for HSV1/2, which contained 1 [micro]l each of HSV1 primer, HSV2
primer, HSV1 probe, and HSV2
Blood samples were tested to detect HAV antibody (Ab), hepatitis E IgG (HEV IgG) and HSV2
IgG, using commercially available ELISA kits (Dia.Pro Diagnostic BioProbes srl, Italy) and rapid plasma reagin (RPR) test for syphilis.
* Patient EBV HHV6B HHV7 CMV, VZV, HSV1, HSV2
, HHV8 Healthy 3 + - + - 9 + - + - 11 - - + - 24 - - + - 32 - - + - Peri-implantitis 2 + - + - 12 - - + - 13 - - + - 25 - - + - 27 - - + - 29 + - + - 37 + - + - 39 + + + - +: positive; -: negative.
The genome of BV (as well as HVP2 and SA8) is very similar to that of HSV2
and ChHV in its genetic organization, with homologs of almost every HSV gene being present in the same order and orientation in BV [77, 92, 99,105,106].
The British researchers who published the study suggest in their paper it contracted HSV2
while feasting on meat of chimpanzee ancestors, the infection carrying over either through bites or through open sores on the body.
The observation of HSV-2 seropositivity in 58.8% among the 51 cases of STIs studied, is in concordance with the data obtained from various STD clinics in India, where HSV2
seroprevalence among high risk group was 20% to 85%.
The patient's HSV-2 IgM antibody test and HSV2
DNA by PCR returned positive and repeat blood cultures grew candida albicans.
These cells stained positively with HSV-1 and HSV2
immunostains (Figures 4(a) and 4(b)).
Although HSV1 is far more common than HSV2
in western populations, a meta-analysis of herpes hepatitis has implicated HSV2
in most cases (HSV1, 35%; HSV2
, 65%) (142); thus, knowledge of antibody specificity or use of cocktails is important in analyzing these severe cases.
For that purpose, we retrospectively screened panels of serum samples that were originally collected over a period of years during the mosquito season and sent to our diagnostic laboratory for detection of antibodies to either causative agents of CNS infection (HSV1, HSV2
, human herpesvirus 6, varicella zoster virus, Mycoplasma pneumoniae), Puumala virus, or INKV.
Darby, "Predicting and preventing the emergence of antiviral drug resistance in HSV2
," Nature Medicine, vol.