immunoblotting


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Related to immunoblotting: immunoprecipitation

im·mu·no·blot

 (ĭm′yə-nō-blŏt′, ĭ-myo͞o′-)
n.
A laboratory procedure, such as Western blot analysis, in which proteins that have been separated by electrophoresis are transferred to a membrane of nitrocellulose or another material and are identified by their reaction with labeled antibodies.
intr. & tr.v. im·mu·no·blot·ted, im·mu·no·blot·ting, im·mu·no·blots
To perform or cause to be subjected to an immunoblot.

immunoblotting

(ˌɪmjʊnəʊˈblɒtɪŋ)
n
the act or process of making immunoblots
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References in periodicals archive ?
In order to investigate tyrosine phosphorylation of Kv channels in PASMCs, fluorescence detection and immunoblotting were performed.
sup][3],[4] More researchers have exploited various techniques, such as immunoblotting, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA), to increase anti-Sm antibody detection sensitivity.
To identify the allergen(s) in the cyanobacteria that is binding to IgE, we performed 2D gel electrophoresis, followed by immunoblotting, using MC(-) lysate and pooled patient sera.
Immunoblotting revealed that the abundance of COX-1 protein was unchanged between the obstructed and the non-obstructed kidney in response to either 9 or 15 weeks of PUUO (Table 2, Fig.
A European multi-center study of immunoblotting in serodiagnosis of Lyme borreliosis.
The primary anti-PCSK9 antibody used for immunoblotting (anti-IB PCSK9 Ab-03) (7) was raised in rabbits against recombinant PCSK9 amino acid residues 31-454 and used at a dilution of 1 part in 2000.
The pellet was resuspended in 100 [micro]l of PBS and used for detection of rL 1 protein by immunoblotting where the reaction signals were detected using the chemiluminescent ECL system following manufacturer's instructions (Amersham, UK).
Scanning densitometry: After separation by SDS-PAGE and immunoblotting, each track of the SDS-PAGE and NCP strips were analyzed by scanning densitometry (CS 9000; Shimadzu Corporation Spectrophotometric Instrument, Japan) at 550nm
However, in the European Union, the Official Method of Analysis is based on the identification of bovine g2-and g3-casein using gel electrophoresis and immunoblotting procedures.
Relative concentrations and localization of placental lactogen-II (PL-II) and the PRL- like protein A (PLP-A) in chorioalantoic placenta were estimated by Immunoblotting and Immunocytochemical analysis.
A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis.