immunoprecipitation

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Related to Immunoprecipitated: Coimmunoprecipitation

im•mu•no•pre•cip•i•ta•tion

(ˌɪm yə noʊ prɪˌsɪp ɪˈteɪ ʃən, ɪˌmyu-)

n.
the separation of an antigen from a solution by the formation of a large complex with its specific antibody.
[1965–70]
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.
References in periodicals archive ?
To demonstrate binding of the antibody specifically to rat [beta]3 (associated with [alpha]V since this represents the active integrin unit), lysates from normoglycemic rat kidney were immunoprecipitated with an anti-[alpha]V antibody and then probed with a biotin-conjugated anti-[beta]3 (C-loop) probe.
Although the lysates used as the input for IP analysis are a postnuclear supernatant, we confirmed that core histone H3 was not detected in the immunoprecipitated complex (IP) indicating that nuclear pools of H1.2 were unlikely to be detected in the immunoblots (Figure 3(a)).
Approximately 0.5 mg of the lysate protein was immunoprecipitated using monoclonal antiserum of c-Met (1 [micro]g) and 15 [micro]1 of protein A/G-agarose (Santa Cruz).
Cell lysate from the labeled cells was treated with trypsin, and both trypsin-treated and intact samples were immunoprecipitated with an anti-[alpha]-Gal A antibody.
HUT-78 cell lysates will be collected, Ikaros protein immunoprecipitated, and two-dimensional electrophoresis gels performed.
Briefly, nuclear lysates from porcine ear feeder cells (PEF cells) were sonicated, after which cross-linked proteins were immunoprecipitated by incubation with antibodies against Sp1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
immunoprecipitated LINE-1 ribonucleoproteins by using FLAG-tagged ORF1p or ORF2p and found that L1 RNA represented 8.3%-10.3% and 18.0%-28.2% of reads associated with ORF1p pulldown or ORF2p pulldown, respectively (31).
(a) Protein samples from control (C) and E4/E4 (E4) neurons, after five weeks in culture, were immunoprecipitated with mouse anti-SORL1 antibody (co-IP SORL1) and analyzed with rabbit anti- APP.
Flag-tagged recombinant protein expressed in 293T cells was immunoprecipitated through anti-Flag antibody conjugated agarose gel (Sigma-Aldrich).
Because multiple bands were detected by [alpha]PC when the MC(-) lysate was used, we asked whether immunoprecipitated phycocyanin could elicit IgE-specific reactivity.