Lueders T, Manefield M, Friedrich MW (2004) Enhanced sensitivity of DNA- and rRNA-based stable isotope probing by fractionation and quantitative analysis of
isopycnic centrifugation gradients.
Fulmer, "Fractionation of rat alveolar macrophages by
isopycnic centrifugation: morphological, cytochemical, biochemical, and functional properties," Journal of Leukocyte Biology, vol.
This [sup.13]C-enriched RNA ("heavy" RNA) can then be separated from unlabeled [sup.12]C background RNA ("light" RNA) using
isopycnic density gradient centrifugation [21, 22].
Under ultracentrifugation conditions, particles having the same buoyant density travel along the axis of the centrifugal force to form an
isopycnic layer.
Takayama and Onodera have studied transmission of underwater spherical shock wave through micro explosion induced by shock of ruby laser to plumbum board, quantitatively describing movement and
isopycnic line of underwater spherical shock wave [5]; Swisdak Jr.
50 gL of rgVEGF164 (1 mg/mL) was applied to a dorsal and lateral area of shaved skin, and control vehicle was applied with
isopycnic phosphate-buffered saline to the opposing adjacent area on the same animal).
Lipoproteins were isolated by
isopycnic ultracentrifugation according to the methods described by Himber et al.
It has evolved from the Miami
Isopycnic Coordinate Ocean Model (MICOM) and is isopycnal in the open stratified ocean but reverts to a terrain-following coordinate in shallow coastal regions, and to z-level coordinates near the surface in the mixed layer.
After
isopycnic centrifugation with Percoll, a portion of the collected cell suspensions (~ 5 x 105 cells) was stained with combinations of fluorochrome-conjugated antibodies against CD11b, CD68, and F4/80: F4/80-PE (Biolegend), CD11b-PerCP-Cy5.5 (BD Biosciences), and CD68Alexa Fluor 684 (AbD Serotec).
(Ah, the correct term is '
isopycnic centrifugation'.) Nothing about other ions.
NV was purified from the stool specimens of infected volunteers by using a CsCl
isopycnic ultracentrifugation gradient as described (5).
SINV-1 purification was accomplished by differential and
isopycnic centrifugation as described previously (Valles and Hashimoto 2009).