The crystalline edifice of bacterial DNA has provided an understanding of the mechanism of mispair recognition by MutS. Each monomer of the homodimer contains six structural domains with discrete functions.
MutS mediates heteroduplex loop formation by a translocation mechanism.
An Arabidopsis thaliana gene that predicts a protein belonging to the MSH2 subfamily of eukaryotic MutS homologs," Plant Physiology, vol.
Hays, "Arabidopsis MutS homologs--AtMSH2, AtMSH3, AtMSH6, and a novel AtMSH7--form three distinct protein heterodimers with different specificities for mismatched DNA," Plant Cell, vol.
Thermus thermophilus MutS (ttMutS) was prepared as previously described .
However, it is known that MutS has a Walker's A-type ATPase motif [14, 15] and that the conformation and function of MutS are greatly affected by the binding and hydrolysis of ATP.