We generated pseudotypes as described (11) by using 25 [micro]g EBOV-GP expression plasmid or 30 [micro]g Machupo virus
glycoprotein (MACV-GP) expression plasmid and 10 pg pNL-Luc or pNL-Ren.
Outside of the the Filoviridae, there are the Arenaviridae (Lassa fever and more exceptionally the Junin and Machupo virus
), and the Bunyaviridae (Crimean-Congo hemorrhagic fever).
In 1963, Machupo virus
was isolated in the remote savannas of Bolivia.
Calomys callosus (or a close relative, see Salazar-Bravo et al., 2002) was identified as the reservoir for Machupo virus
, the etiological agent for Bolivian Hemorrhagic Fever (Johnson et al., 1966).
In July 1934, an outbreak of Bolivian hemorrhagic fever (BHF), which is caused by Machupo virus
, began in northeastern Bolivia.
Tacaribe serocomplex viruses (family Arenaviridae, genus Arenavirus) comprise Bear Canyon virus, Tamiami virus, and Whitewater Arroyo virus in the United States; Tacaribe virus (TCRV) on Trinidad; Chapare virus (CHPV) and Machupo virus
(MACV) in Bolivia; Guanarito virus (GTOV) in Venezuela; Junin virus (JUNV) in Argentina; Sabia virus (SABV) in Brazil; and 9 other species (1).
These agents are members of several virus groups, including filoviruses (Ebola virus, Marburg virus), Old World arenaviruses (Lassa virus, Lujo virus), New World arenaviruses (Machupo virus
, Junin virus, Sabia virus, Guanarito virus, Chapare virus), flaviviruses (Omsk hemorrhagic fever virus, Kyasanur Forest disease virus), and bunyaviruses (Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus) (1).
However, it was not until 1963 that the etiologic agent, Machupo virus
, was isolated from the spleen of a patient who died from this disease (1).