Alternatively, most genes (usually approximately 2000 genes per bacterium) in the microbiome are sequenced so that metabolic and other functional pathways can be evaluated in each individual's metagenome
. Functional WG data provide opportunities to find out which metabolic pathways are affected and how the microbiome may contribute mechanistically to health and disease states.
Such tools include ARB-Silva, a curated database of full-length high-quality 16S rRNA gene sequences including those from genome and metagenome
studies (Pruesse et at, 2007).
KEY WORDS: bacteria, bacterial metagenome
, cigarettes, pathogens, smoking, tobacco.
of YN3 harbored 18 rdhA genes (designated YN3rdhA1-18) encoding the catalytic subunit of reductive dehalogenase (rdhA), four of which were suggested to be involved in PCE-to-ETH dechlorination based on significant increases in their transcription in response to CE addition.
Use of specific primers to amplify bacterial ribosomal genes from the metagenome
and analyze them using DGGE to understand microbial diversity is known to be very useful culture independent approach.
analysis without the PCR amplification step can be a powerful alternative to amplicon-based profiling for investigating all metabolic processes of microorganisms found in environmental samples.
analysis of an extreme microbial symbiosis reveals eurythermal adaptation and metabolic flexibility.Proc.Natl.
Dysbiosis is a form of altered gut metagenome
and collected microbial activities, and, in combination with classic genetic and environmental factors, it may promote the development of metabolic disorders .
Loman et al., "Extensive modulation of the fecal metagenome
in children with Crohn's disease during exclusive enteral nutrition," American Journal of Gastroenterology, vol.