Further, sodium metaperiodate
solution (40 mM in 62.5 mM [H.sub.2]S[O.sub.4],0.25 mL) was added to the supernatant (0.5 mL), and the mixture was allowed to stand for 20 min at room temperature.
As described previously , kidney tissue sections were incubated in saturated sodium metaperiodate
The rats were anesthetized with Nembutal (30 mg/kg) at the end of each experimental schedule, perfused intracardially with 0.01 M phosphate-buffered saline (PBS, pH 7.4), and fixed in PLP fixative (0.01 M sodium metaperiodate
; 0.075 M L-lysine-HCl; 2% paraformaldehyde, 0.03 M PB, pH 6.2).
The gingival samples were immersed in a periodate lysine paraformaldehyde (PLP) fixative consisting of 0.01 M sodium metaperiodate
, 0.075 M L-lysine-HCL, 4% paraformaldehyde, and 0.03% phosphate buffer (pH 6.2) for 6 h at 4[degrees]C.
Khedr, "Spectrophotometric determination of some catecholamine drugs using metaperiodate
," Journal of the Association of Official Analytical Chemists, vol.
We also subjected the alginate to the oxidation process with sodium metaperiodate
, following a partial oxidation procedure.
Gingival samples were immersed in the periodate lysine paraformaldehyde (PLP) fixative consisting of 0.01 M sodium metaperiodate
, 0.075 M L-lysine-HCl, 4% paraformaldehyde, and 0.03% phosphate buffer (pH 6.2) for 6h at 4[degrees]C.
Sodium alginate (medium viscosity grade, viscosity of 2% solution: 3500 cps at 25[degrees]C, gelatin (Bloom 300, Type A), sodium metaperiodate
and sodium tetraborate decahydrate (borax) were obtained from Sigma Chemicals Co., St.
seeds (Abdel & Smith, 1951) was dissolved in 100 ml water and sodium metaperiodate
solution (0.025M, 100ml) and make up the volume to 250ml with distilled water.
This is supported by the works of Albuquerque and colleagues (2005) and Ferreira and coworker (2008); who worked with 43gp antigen treated with sodium metaperiodate
to eliminated the carbohydrate epitopes responsible for cross reactions between PCM and histoplasmosis sera.
Boric acid, guanidine hydrochloride, sodium metaperiodate
, and acetonitrile were purchased from Wako Pure Chemicals Co.