An aliquot (0.1 ml) from microbial suspension was spread onto solidified agar medium (15 ml) in petri dish (Muller-Hinton Agar
(MHA, Oxoid) for bacterial cultures and Saboraud Dextrose Agar (SDA) for fungal cultures.
Poor sensitivity in DDST mainly due to the lack of optimum disc space results in the impaired ESBLs inhibition by clavulanate leads to the inaccurate identification of chromosomal cephalosporinases producing ESBLs strain in the Muller-Hinton agar
MRSA was detected by using cefoxitin (30ug) disc on Muller-Hinton agar
(MHA) as per CLSI 2014 guidelines8.
In Muller-Hinton agar
plates supplemented with 4% NaCl, the test strain was lawn cultured, oxacillin E strip was placed on the medium and incubated for 24 h at 35[degrees]C.
Bacterial cultures were maintained on nutrient Muller-Hinton agar
at 37[degrees]C, and the cultures were kept in appropriate media slants and stored at 4[degrees]C until used.
The tested microorganisms were separately cultured on sterilized Muller-Hinton Agar
(MHA) at 37[degrees]C for 24 hours by using streak plate method.
We determined MIC by using the Etest method with Muller-Hinton agar
containing 25 mg/L G6P.
Susceptibility to antimicrobial agents was determined both by disk diffusion method of Kirby-Bauer and minimum inhibitory concentration (MIC) method on Muller-Hinton agar
as described by the Clinical Laboratory Standard Institute (CLSI) 2014.12 Antibiotics used for antibiogram determination of the collected strains among FQ were: norfloxacin, ciprofloxacin, ofloxacin, enoxacin, moxifloxacin, and sparfloxacin and nalidixic acid.
Antibacterial activity of the extracts was determined by disc diffusion method on Muller-Hinton agar
(MHA) medium in comparison with standard antibiotic ampicillin (20 [micro]l/disc).
The isolates were subjected to cefpodoxime (10 [micro]g), ceftazidime (30 [micro]g), and cefotaxime (30 [micro]g) antibiotic disks alone and in combination with clavulanic acid (10 [micro]g) (Oxoid; Basingstoke, UK) to determine the presence of ESBLs by the disc diffusion method on Muller-Hinton agar
plates, using respective bacterial suspensions with the turbidity adjusted to a 0.5 McFarland standard.
First, in Europe, EUCAST propagates Muller-Hinton agar
with horse blood for susceptibility testing of fastidious organisms (like S.
Briefly, Muller-Hinton agar
was hydrated (77%), sterilized, and mixed with 1 mL of nanoparticle solution.