After decomposition of the metal complex in hot concentrated nitric acid and neutralization of the reaction medium the resulting metal ions were determined complexmetrically using EDTA and murexide
or Eriochrome Black T (EBT) indicator system.
Then 10 [cm.sup.3] of the solution was drawn; the sample was alkalized with concentrated aqua ammonia and titrated with 0.05 mol [dm.sup.-3] EDTA against murexide
. Analyses were performed duplicate with standard deviation not exceeding 1.0%.
The nickel content in the solution was determined by complexometric titration in the presence of the indicator murexide
Determination of Calcium using Murexide
as an Indicator The determination of Ca in the sample was based upon the principle of titration.
The chelating activity of zinc ions for P australis leaf extracts solution 1mg/ml or 10mg/ml in DMSO for Zn and Cu chelating activity respectively was measured by the method of murexide
described by Watak and patil, (2012) with slight modification.
The samples were analyzed within 72 hr after collection by standard protocol (Eaton et al., 2005) for pH (Orion J Star pH Benchtop, Thermo Scientific, Singapore), electrical conductance (Orion 3 Star Conductivity Benchtop, Thermo Scientific, Singapore), total alkalinity (acidimetry to pH 4.5 endpoint), total hardness (versenate titration to eriochrome black-T endpoint) and calcium hardness (precipitation of magnesium followed by versenate titration to the murexide
Analysis of urates: The white substance accumulated in the pericardium was collected and analyzed via the Murexide
A known amount of plant material was demosturized by placing it in an oven at 105C till constant weight was obtained and then this material was placed in covered crucible and ashed in furnace at 550C for 5 h then dissolved this ash in the concentrated nitric acid for determination of mineral contents.Amount of Ca and Mg was determined by titration method using murexide
and Eriochrome black T indicator respectively.
Removal of Murexide
(Dye) from Aqueous Media using Rice Husk as an Adsorbent.
The phosphorus contents in SBF solutions were determined with a UV mini 1240 spectrophotometer (SHIMADZU) as reported by Murphy and Riley , and the calcium concentration was determined by volumetric titration using NaOH, Murexide
and EDTA (from Aldrich).
test differentiates urate deposits and calcium deposits.