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13.3 ND Not identified Dematiaceous fungi 53.3 29.4 Mycelia sterilia 46.7 23.5 Regions of Rio Grande do Sul State, Brazil North western South western (n= 16) (n= 4) % [bar.x] Infection 72.5 (ab) (40 - 100) 50.0a (35 - 55) (range) [bar.x] Aw (range) 0.737 (ab) (0.71 - 0.77) 0.721a (0.68 - 0.75) Filamentous fungi FO (%) FO (%) Alternaria sp.
Non sporulating cultures were distinguished from each other by their cultural characteristics such as colony morphology, hyphal mat characteristics and pigmentation of the colony in the medium and were grouped under Mycelia sterilia 28.
The identified species were grouped as follows: 149 Ascomycota, one Basidiomycota and 52 Mycelia sterilia. Hyphomicetous asexual species of Ascomycota (108 species) dominated, while Coelomycetous species represented 21% of the isolates (38 species).
Amplification products of ITS regions from 40 Mycelia sterilia were analyzed through PCR-RFLP (PCR-restriction fragment length polymorphism).
On Code Outer Code 1st 1st 2nd Alternaria alternata 1 Aspergillus flavo Aspergillus fumigatus 1 Aspergillus nidulans Aspergillus niger 1 1 Aspergillus terreus 8 7 Aspergillus versicolor 4 Eurotium amstelodami 1 Eurotium repens 1 Paecilomyces variotii 1 Penicillium chrysogenum 2 Penicillium expansum 2 Saccharomycetaceae 1 Mycelia sterilia hyaline 1 Total CFU 4 16 12 Control Total 1st 2nd Alternaria alternata 1 Aspergillus flavo 1 1 Aspergillus fumigatus 1 Aspergillus nidulans 1 1 Aspergillus niger 2 Aspergillus terreus 1 16 Aspergillus versicolor 1 5 Eurotium amstelodami 1 Eurotium repens 1 Paecilomyces variotii 1 Penicillium chrysogenum 2 Penicillium expansum 2 Saccharomycetaceae 1 Mycelia sterilia hyaline 1 Total CFU 3 1 36 Table 2: 16S rDNA-based identification of bacterial isolates.
It was used for identification of Rhizopus species; "Mucorales" Zycha, [47] for Aspergillus species;" The Genus Aspergillus" [35] and "List of accepted species and their synonyms in the family Trichocomonaceae" Pitt, [33] for Penicillium, Gliocladium, Paecilomyces species; A Manual of the Penicillia" [36], for Alternaria, Cladosporium, Ulocladium, Aureobasidium, Scopulariopsis species; "Dematiaceous Hypomycetes" Ellis, [20] and Simmons [40], for Fusarium species; " The Genus Fusarium" Booth, [11] for Mycelia sterilia; "Ainsworth & Bisby's Dictionary of the Fungi" Ainsworth, [2] and "Tohumsuz Bitkiler Sistematigi" (Bacteriophyta, Cyanophyta, Phycophyta, Mycophyta, Lichenes) [19].