We prepared transverse slices of the nasopalatal region from GD 11.5 embryos (n [greater than or equal to] 3 for each condition) from which the eyes and the maxillary component of first branchial arches were removed.
In agreement with the lack of effect of TCDD on transgene activity, we did not detect a significant difference for any of these four genes in the nasopalatal region of TCDD-treated (n = 3) or control (n = 3) embryos.
During this period of development, RALDH3 is the sole RA-synthesizing enzyme expressed in the nasopalatal region as assessed by in situ hybridization (Figure 4A) and by the complete disappearance of Tg(RARE-Hspalb/lacZ)l2Jrt activity in [Aldhla3.sup.-/-] fetuses (Figure 4C).
Of the 33 patients for whom clinical data were available, 25 (76%) had [greater than or equal to] 1 complication, including 15 patients with erythema nodosum leprosum (ENL) and 3 with severe facial involvement (lagophthalmos, blindness,
nasopalatal destruction).