FGF signaling is required for determination of otic neuroblasts
in the chick embryo.
Instead, the investigators intended to use hypothermia (cooling the animals to 1-4[degrees]C by laying them on ice for about six minutes) to immobilize neonatal mice and rats while carrying out intracranial injections of virions or neuroblasts
Neuroblastoma usually affects babies and children and forms in certain types of nerve tissue called neuroblasts
should be negative in order to make this diagnosis.
As a model system for understanding cancellation mechanisms of biological noise in a multicellular organism, the researchers looked at a particular development stage of the visual system in fruit flies: the propagation of the so-called "proneural wave." during which cells of a type called neuroepithelial cell differentiate into neuroblasts
(cells in the process of dividing into neurons).
A: Low-power histology of the mass (hematoxylin and eosin stain, original magnification x4) shows a nodular proliferation of small neuroblasts
in a background of fibrillary neuropil.
In the vehicle-treated group, DCX + cells (29.0 [+ or -] 4.5 cells) as neuroblasts
were easily found in the SGZ of the DG [Figure 2]A.
Bertet et al., "Temporal patterning of Drosophila medulla neuroblasts
controls neural fates," Nature, vol.
Anti-Ki67 (1:200, Abcam, UK), antidoublecortin (DCX) (1: 100, Santa Cruz, USA), anticollagen IV (1 : 200, Abcam, UK), anti-CD31 (1 : 200, BD Biosciences, USA), anti-Iba1 (1 : 200, Wako, Japan), and anti-GFAP (1:400, Millipore, Burlington, USA) antibodies were used to detect proliferative cells, neuroblasts
, basal lamina of endothelial cells, endothelial cells, microglia/ macrophage cells, and astrocytes, respectively.
Heuckeroth, "Enteric neuroblasts
require the phosphatidylinositol 3-kinase/Akt/Forkhead pathway for GDNF-stimulated survival," Molecular and Cellular Neuroscience, vol.
Most of the cases reported in the elderly (including ours) occurred in the hemisphere of the cerebellum, presumably due to migration of the undifferentiated neuroblasts
from the posterior medullary velum laterally .