67 - SYNTHESIS AND CHARACTERIZATION OF NITROSYLATED
TRIS-(ETHYLENEDIAMINE) COBALT (III) COMPLEX.
COX-2 produces epi-lipoxin A4 (epi-[LXA.sub.4]), a potent anti-inflammatory mediator and competitor ligand of SAA for their common [LXA.sub.4] receptor, ALX/FPR2 [48, 49].
(47) Indeed, our results indicated that TRPV1 is nitrosylated
. TRPV1 also shows sensitivity to phenylarsine oxide and allicin from garlic through the covalent modification of Cys residues located in the C-terminal and N-terminal regions.
The cerebral cortex and cerebellum also responded with a slower increase of TNF[alpha], but these regions did not show increases in nitrosylated
proteins or oxidized lipids at any time.
NO can also be released from synthetic NO-donor organic compounds, prominent examples are organic nitrates, furozans, hydroxyguanidines, diazeniumdiolates, S-nitrosothiols, nitrosylated
metal complexes, and others [14, 15].
The lower Figure 9B shows the number of nitrosylated
groups per insulin molecules analyzed by densitometry.
membrane proteins are located in bands which migrate at 60-90 kDa, 40-60 kDa, and 10-20 kDa.
(2009) who found increased serum concentrations of nitrosylated
proteins (biomarkers of OxS) in nickel-allergic female patients after oral nickel challenge .
The quantitative measurement of nitrosylated
protein can be done using the nitrotyrosine ELISA kit.
(39-41) Indeed, the presence of nitrosylated
tyrosine residues (Nitro tyrosine) has been recognized as an indicator of cell damage and inflammation, as well as of the production of ONOO.
Nevertheless, elevated peroxynitrite levels may be one of the causative factors, and reduced elimination of free cholesterol by nitrosylated
Apoprotein A1 with elevated peroxinitrite (Shao et al., 2005) may account for the increased free cholesterol in the tubule cells of exhausted rats.
New techniques that are suitable for the diagnostic analysis of nitrosylated
proteins are now available and capable to reveal NO targets in plants.