47) Indeed, our results indicated that TRPV1 is nitrosylated
The cerebral cortex and cerebellum also responded with a slower increase of TNF[alpha], but these regions did not show increases in nitrosylated
proteins or oxidized lipids at any time.
NO can also be released from synthetic NO-donor organic compounds, prominent examples are organic nitrates, furozans, hydroxyguanidines, diazeniumdiolates, S-nitrosothiols, nitrosylated
metal complexes, and others [14, 15].
Nevertheless, it is to note that nitrosylated
proteins include cytoskeleton, cell migration, cell cycle, and antiapoptotic proteins, as well as proteins involved in transcription and protein synthesis [65-69].
The lower Figure 9B shows the number of nitrosylated
groups per insulin molecules analyzed by densitometry.
membrane proteins are located in bands which migrate at 60-90 kDa, 40-60 kDa, and 10-20 kDa.
The quantitative measurement of nitrosylated
protein can be done using the nitrotyrosine ELISA kit.
39-41) Indeed, the presence of nitrosylated
tyrosine residues (Nitro tyrosine) has been recognized as an indicator of cell damage and inflammation, as well as of the production of ONOO.
Nevertheless, elevated peroxynitrite levels may be one of the causative factors, and reduced elimination of free cholesterol by nitrosylated
Apoprotein A1 with elevated peroxinitrite (Shao et al.