Northern blot analysis


Also found in: Thesaurus, Medical, Encyclopedia.

Northern blot analysis

n.
A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to a nylon or nitrocellulose membrane, and identified with a suitable probe, such as an RNA fragment.

[Playful coinage after Southern blot analysis.]
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.
Mentioned in ?
References in periodicals archive ?
Expression of transforming growth factor alpha in human tissues: Immunohistochemical study and Northern blot analysis. Virchows Arch.
RNA Extraction and Northern Blot Analysis. Total RNA was extracted from leaf of transgenic rice plants using TRIzol[R] Reagent (Thermo Fisher Scientific) following the manufacturer's recommendation.
In order to assess cell binding and internalization of the selected aptamers without the need for labeling, we performed Northern blot analysis. MiaPaCa-2 cells were incubated with the aptamers, and total RNA isolated from the cells were subjected to Northern blot analysis by using a 5'-[[[gamma]-.sup.32]P]-radiolabeled probe (BW28) that was complementary to the 3' constant region of the aptamer library (Figure 3(a)).
Northern blot analysis of ventricular and skeletal Mb mRNA levels of intact rats from postnatal (P) day 7 through P42 is shown at the top of the figure, and the quantitative results obtained by densitometric analysis of Mb and 18s rRNA transcripts hybridization ratio are shown at the bottom, in arbitrary units (A.U.).
coli Total RNA Extraction and Northern Blot Analysis. After solubilization, cells were solubilized in Eurosol (EuroClone) solution and chloroform was added in the proportion of 1: 10.
For Northern blot analysis, total RNA was extracted from leaves using Trizol reagent (Invitrogen), and hybridizations were carried out with [[[alpha].sup.32] P] dCTP-labeled PHYB probe.
Northern Blot Analysis. Northern blot analysis was performed as previously described [10].
Expression of NQO-1, GST-alpha, gamma-glutamylcysteine synthetase-heavy and -light chains, and microsomal GST was assessed by Northern blot analysis. Sulforaphane and broccoli sprout extract potently induce quinone reductase activity in cultured prostate cells, and this induction appears to be mediated by increased transcription of the NQO-1 gene.
For three miRNA 161, 168a&b and 171a a good correlation between the array and Northern blot analysis was observed; i.e.
Northern blot analysis has shown that M46 is expressed in G186AS and Downs strains, but is transcriptionally silent in G184AS and G217B strains.
For Northern blot analysis, l0[micro]g of total RNA of each sample was denatured by incubation for 1 h at 50 [degrees]C in a solution of 0.1mol/l of sodium phosphate buffer (pH 7.0), 50% of dimethyl sulfoxide, 15% of deionized glyoxal, and 0.3% of RNA dye.

Full browser ?