5% buffered gluteraldehyde and then cut in cubes of 1 mm3 before placed in 1% OsO4
for contrasting for at least one hour.
Post fixing was done with Osmium tetroxide2Oso4
prepared as 2% aqueous solution (1 g of Oso4
in 50 ml D.
Se tomaron muestras de placa alba empleando un palillo esteril de madera y se suspendieron en solucion fijadora de Karnovski (2,5 % glutaraldehido y 2 % paraformaldehido en amortiguador de fosfatos) y se procesaron para microscopia electronica (ME) siguiendo un proceso rapido empleando irradiacion en un horno domestico de microondas (8), que consistio en periodos de irradiacion de 2 minutos a baja potencia (100 W), para los lavados, post-fijacion en OsO4
y deshidratacion en una gradiente de alcohol etilico.
2) and post-fixed for 1 h at room temperature in 1% OsO4
in the 100 mM phosphate buffer.
The enone 5 is a C-nor steroid that may be extended to six membered by dihydroxylation with OsO4
followed by ring cleavage with Pb(OAc)4, which upon intramolecular condensation of resulting trione shall afford 4.
Specimens were then fixed in 1 (Percent) OsO4
for 1 h.
After washing with phosphate buffer, the tissue was treated for 1 h with 1% OsO4
in phosphate buffer and then dehydrated in an ethanol series 25, 50, 75 and 100 %, one h for every line, Then dried for 2.
The observation of SEM images of two herbal tissues stabilized by using 1% solution of OsO4
and the obtained nanocomposite clearly showed that the magnification of images in the sample stabilized with the nanocomposite increased significantly.
3, and fixed in 1% solution of OsO4
(Bozzola & Russell, 1992; Ruzin, 1999).
Specimens for transmission electron (TEM) and optical microscopy were completely decalcified, postfixed in OsO4
(2%) for 2 h at 4 [degrees]C, and embedded in epoxy resin Aname Epon 812 (EMS).