polymerase chain reaction

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polymerase chain reaction

n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.

pol′ymerase chain′ reac`tion



n.
the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
Translations
amplification en chaîne par polymérase
References in periodicals archive ?
Contract awarded for Delivery of a set for dna isolation from food and feed as well as a reagent to carry out a real time pcr reaction for the department of veterinary hygiene in warsaw.
The PCR reaction was performed using 2.5 [micro]L 10X buffer (MBI Fermentas), 1 [micro]L of 2.5 mM dNTP (Invitrogen, USA), 1.5 [micro]L of 25 mM MgCE (MBI Fermentas), 2 [micro]L of the 10 pmol EI1 primer (Invitrogen, USA), 0.2 [micro]L of 1.25 U taq DNA polymerase (MBI Fermentas) and 1.0 [micro]L of DNA.
If we conduct a standard PCR reaction with these primers and have any circularized padlock probe present, consider what happens.
Droplet digital PCR reaction was prepared as previously described (28) to quantify the mutation abundance of DNA samples before and after ND-NaME-PrO mutation enrichment.
Adenovirus reaction was performed in a total 45 [micro]l reaction volume using 2 [micro]l sample supernatant, 3 [micro]l of Taq mixture, and 40 [micro]l of ADV PCR reaction solution.
The assay format employed 2 parallel PCR reactions using extracted RNA from a clinical sample in a real-time PCR reaction.
The PCR reaction mixture was set up by taking 5 [micron]L of the genomic DNA, 12.5 [micron]L of 2 x PCR master mix, 1 [micron]L of each primers (10 pmol) and balanced with nuclease free water for 25 [micron]L of total PCR reaction volume.
The 50 [micro]L PCR reaction contained 1x HF buffer, 200 [micro]M of dNTP, forward and reverse primers at 200 nM each, 1 unit of Phusion enzyme, and 5 [micro]L RT-PCR amplicon.
This is an important issue because the efficiency of the PCR reaction should be between 90-100% (slope between 3.32 and 3.6) (21).
Primers pairs and probes for cDNA synthesis was designed and Real time PCR reaction were performed.