5 g) was digested at 150degC in diacid mixture (nitric acid and perchloric
acid) following Miller (1998).
We purchased HPLC-grade methanol, acetonitrile, acetic acid, ammonium acetate, sodium tungstate, and perchloric
acid from Merck.
acid and radioisotope hoods and biological safety cabinets are generally segregated from general chemical exhaust due to incompatibility or special operating conditions.
For differentiation between bacterial supernatant and bacterial sediment, 100 [micro]l/ml perchloric
acid (333 mmol/l stock solution) was added to precipitate protein.
1 M perchloric
acid, and an aliquot of homogenate (50 [micro]L) was assayed for protein (Lowry et al.
EPA, with assistance from the Bureau of Alcohol, Tobacco, and Firearms (ATF) and the Knoxville Bomb Squad, detonated shook sensitive and reactive materials which included perchloric
acid, nitrates, sodium hypophosphate and picric acid.
The effects of stirring speed, leaching temperature, perchloric
acid concentration, solid/liquid ratio and particle size on conventional leaching were determined.
The organ samples were digested in a mixture of nitric acid (HNO3) and perchloric
acid (HClO4) (Ebrahimpour and Mushrifah, 2010a; Mansouri et al .
Instead of putting 10ml nitric acid (55%) and 5ml perchloric
acid (70%) at the time of digestion, 5ml nitric acid (55%) and 2ml perchloric
acid (70%) were added to each flask and the flasks then were kept air tight overnight.
The use of potassium dichromate solvated in dilute perchloric
acid is an established method for validating the absorbance scale and linearity of a spectrophotometer in the UV region.
Formation of hydrogen peroxide during precipitation of red cells with perchloric
acid: a cautionary note for precise determination of pyruvate, GSH and NAD(P)H.
05 M perchloric
acid containing 10 nmol EHC as an internal standard (IS) using an ultrasonic cell disruptor (US-300T, Nissei, Tokyo, Japan).