It is only when a cytosine occurs in sequence next to a guanine (so-called CpG island--where the -p- represents the phosphodiester
link between the C and G bases), that such a methylation is possible.
Structurally, phosphatidylserine is an amino acid that consists of glycerophosphate skeleton conjugated with two fatty acids and L-serine via a phosphodiester
During early 1990s, Gabibov and colleagues reported that autoantibodies purified from the sera of patients with SLE and other autoimmune diseases cleave phosphodiester
bonds (2, 38, 39).
Phytase (Myo-inositol hexakisphosphate phosphohydrolase) can hydrolyze the phosphodiester
bonds of phytic acid, producing inorganic phosphate and phosphate ester (1).
Synthesis and anti-HIV Evaluation of Alkyl and Alkoxyethyl Phosphodiester
These enzymes catalyze the reaction of nucleic acid phosphodiester
bond hydrolysis at the 5 'end of the oxidized nitrogen base, while the 3'-hydroxyl and 5'-phosphate ends remain free.
If the padlock probe is mixed with target nucleic acids containing these sequence complements, thermally denatured and reannealed, it can hybridize down to its target at both ends, effectively circularizing the probe and placing its 3' and 5' ends immediately next to each other, but with a nick--that is, missing the phosphodiester
bond; see Figure lb.
The lack of major shining of the A-RNA marker bands at 815-809 c[m.sup.-1] (phosphodiester
), 867-861 (ribosephosphate), 1247-1241 (phosphate), and 1700-1688 (guanine) indicates that RNA remains in a conformation upon HBCD complexation (Figure 1) (43-45).
Forsyth et al., "Phosphodiester
cleavage properties of copper(II) complexes of 1, 4, 7-triazacyclononane ligands bearing single alkyl guanidine pendants," Inorganic Chemistry, vol.
Thomas, "Selectivity of polyamines on the stability of RNA-DNA hybrids containing phosphodiester
and phosphorothioate oligodeoxyribonucleotides," Biochemistry, vol.
Conventional phosphorylation could produce monosubstituted phosphate monoester starch or cross-linked phosphodiester