The NLRP3 inflammasome is a multiprotein complex composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC; also known as PYCARD
), the effector cysteine protease 1 (caspase 1), and NLRP3.
Deeg, "Stroma-dependent apoptosis in clonal hematopoietic precursors correlates with expression of PYCARD
," Blood, vol.
Once activated, it oligomerizes and recruits the PYD-CARD adaptor protein apoptosis associated speck-like protein containing a CARD (PYCARD
or ASC) and the protease procaspase-1.
DSW 600 downregulated expression of apoptosis inhibitor 5 BCL2- associated athanogene (Api5), cell death- inducing DFFA-like effector b (Ciedb), cytochrome c, and somatic (Cycs), Fas (TNF receptor superfamily, member 6), growth arrest and mitogen activated protein kinase 1 (Mapk1), PYD and CARD domain containing (Pycard
Remarkably, microarray analysis after LincRNA-EPS overexpression highlighted the repression of many proapoptotic genes, such as Bad, Bax, Caspase-2 and-6, Fadd, Pycard
, and with Pycard
being the most affected.
DNA Methylation and Patient Outcome.--DNA methylation status of individual genes, such as those encoding a transcriptional repressor (HIC1), a proapoptotic protein (PYCARD
), a tumor suppressor (LZTS1), and a transporter (SLC6A20), has been associated with either a good or poor prognosis.
After splitting the SSB-bound DNA into 10 different tubes, we still had sufficient material for successful MSP analysis of the CDKN2A, CDKN2B [cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)], and PYCARD
(PYD and CARD domain containing; also known as ASC and TMS1) promoters (data not shown).