The provided abundant recombinant Sudan ebolavirus antibodies target at the L (an RNA-dependent RNA polymerase
and a component of viral polymerase complex) and GP (toxic protein) of EBOV, of which the peptide sequences correspond to the important amio acid residues like TRSFTTHFL, MSDIFHALV, GFKLRSAVM, and TTLPSQNST, etc.
II (RNAPII), the enzyme that produces RNA from a DNA template, can become stuck due to damage to the DNA template, and these jams must be cleared to restore gene expression and normal cell function.
Sofosbuvir, a nucleotide inhibitor of viral NS5B RNA polymerase
has been approved for the treatment of chronic hepatitis C, in combination with other drugs and its approval represents the first key step towards the new era in the treatment of CHC patients, since it is the first approved DAA with potent activity and high genetic barrier against all HCV genotypes13.
The first step in gene expression is the binding to DNA of an enzyme called RNA polymerase
. This binding occurs at very particular sites of the DNA, that are "recognized"by the RNA polymerase
His research focuses on the mechanism of transcription by RNA polymerase
He identified several subunits of a protein complex called RNA polymerase
III--which plays an important role in copying DNA into RNA--among those that were affected.
Adding (literally) a layer of complexity here is that promoters don't directly bind RNA polymerase
. Instead, they contain shorter sub-sequences, which are recognized as binding sites for a class of proteins known as transcription factors (TFs); there are a great many of these, each with their own preferred DNA sequence binding site (usually short, 10-20 base pairs) and their own level of ability to recruit in RNA polymerase
The technique is used to study biological systems, including DNA and molecular motors like RNA polymerase
, an enzyme responsible for copying DNA.
This volume reviews the features of cyanobacterial genetic systems responsible for acclimation to a changing environment, including the two-component regulatory system, eukaryotic-type serine-threonine protein kinases, sigma subunits of RNA polymerase
, transcription factors, and other regulators and gene expression in response to various factors.
Vannini explained that RNA polymerase
and its helper molecules are "often overactive in cancer because cancer cells rely on a large number of protein building blocks to rapidly grow and divide."
Promoter-specific mRNA synthesis requires a minimal set of proteins comprising Pol II (RNA polymerase
II) and associated GTFs (general transcription factors), which is defined as in vitro "basal transcription" .