reverse transcriptase

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reverse transcriptase

n.
A polymerase that catalyzes the formation of DNA on an RNA template, found in the retroviruses and in certain other viruses, such as hepatitis B virus.

reverse transcriptase

(trænˈskrɪpteɪz)
n
(Biochemistry) an enzyme present in retroviruses that copies RNA into DNA, thus reversing the usual flow of genetic information in which DNA is copied into RNA

reverse′ tran•scrip′tase

(trænˈskrɪp teɪs, -teɪz)
n.
a retrovirus enzyme that synthesizes DNA from viral RNA, the reverse of the usual DNA-to-RNA replication: used in genetic engineering.
[1970–75]
ThesaurusAntonymsRelated WordsSynonymsLegend:
Noun1.reverse transcriptase - a polymerase that catalyzes the formation of DNA using RNA as a template; found especially in retroviruses
polymerase - an enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA
References in periodicals archive ?
RNA was reverse transcribed by using the SuperScript II Reverse Transcription Kit (Invitrogen, Carlsbad, CA, USA) and subjected to PCRs specific for DENV (5) CHIKV (6), WNV (7) and Mayaro virus (8).
Because of difficulties with reverse transcribing the entire SINV-3 genome (wild type or recombinant), 3 regions of the SINV-3 genome were reverse transcribed and amplified to verify transcript production.
After 48 h stimulation and 24 h recovery, mRNA was extracted and reverse transcribed.
A band corresponding to ~1117 bp is seen (Figure 3, panel A, lane 3) when RNA extracted from null cells of Dictyostelium was reverse transcribed and subjected to PCR amplification.
Skeletal muscle RNA samples from 15-20 week old WKY and SHR/y rats were reverse transcribed to generate cDNA.
The mRNA for these enzymes was assessed by quantitative reverse transcribed PCR in non-irradiated cells to establish a baseline level.
Total RNA (1 [micro]g) was reverse transcribed and then amplified (30 cycles) by PCR using a Super Script preamplification system for first-strand cDNA synthesis and Taq DNA polymerase (Life Technologies, GIBCO BRL, Rockville, MD, USA).

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