Glycerol, mannitol and sorbitol concentrations increased significantly in infected berries along with free galacturonic acid, arabinose, galactose, rhamnose
, mannose and xylose.
It was extracted with EtOAc and the residue recovered from the organic phase was found to be dibenzofruran while the aqueous phase was concentrated and purified by preparative TLC using the solvent system (EtOAc-MeOH-H2O-HOAc; 4: 2: 2: 2) and identified as D-glucose and L-rhamnose
by comparative TLC of the hydrolyzed sugars with the authentic samples of glucose and rhamnose
and their optical rotation values [[alpha]]D20 + 50.
The standards rhamnose
, fucose, arabinose, xylose, mannose, galactose and glucose were obtained from Merck, together with hydrochloric acid, sulphuric acid, acetic acid, acetic acid anhydride, ammonium hydroxide, potassium hydroxide, sodium sulphate, potassium borohydride and 1-methylimidazol.
Strains LTG7, I5, and G could ferment rhamnose
while strains LCG3 and LI3 could not.
Biochemical characterization of strain B14 Characteristic Strain B14 Colony pigmentation Creamy Motility Utilization of: + Lactose + Xylose + Maltose + Fructose + Dextrose + Galactose + Raffinose + Trihalose + Melobiose + Sucrose + L-arabinose + Mannose - Inulin + Sodium gluconate + Glycerol + Salicin - Dulcitol - Inositol - Sorbitol - Mannitol + Adonitol - Arabitol - Erythritol - a-methyl-D-glucoside - Rhamnose
- Cellobiose + Melezitose + a-methyl-D-mannoside - Xylitol - D-arabinose + Citrate - Malonate - Sorbose - ONPG hydrolysis + Esculin hydrolysis + Table 2.
Fucoidans are complex sulfated polysaccharides consisting mainly of sulfated L-fucose and variable proportions of galactose, mannose, xylose, glucose, rhamnose
and uronic acids (Usov & Bilan, 2009).
5%NaCl [7,8] and characteristically, catalase positive, cytochrome oxidase negative, glucose fermentative, indole positive, citrate negative, lysine positive, mannitol, dulcitol, sorbitol, inositol, xylose, rhamnose
negative, produce hydrogen sulfide, alkaline slant and acid but on Triple sugar iron Agar .
Its fruit has a high content of flavonoids such as rutin, quercetin, and rhamnose
sugar, which form the basis of many pharmacological products such as eye drops (MENDES et al.
Enriched with Rhamnose
, a naturally derived sugar which helps to stimulate activity deep within the skin, the LiftActiv range offers an advanced generation of skin care for anti-wrinkle and firming action.
2007), saponin, alkaloids, glucose, xylose, rhamnose
, arabinose, lupeol, phytosterols, lipids, calcium oxalate crystals and starch (Jiag et al.
biloba are: flavonol-gucoside of kaempferol, flavones, quercetin and isohamnetin with glucose or rhamnose
5 ul/litre) blood agar and fermentation of sugars (L-arabinose, glucose, lactose, maltose, mannose, raffinose, salicin, D-sorbitol, starch, mannitol, rhamnose
and sucrose) were performed according to standards procedures (Holt, 1986).