Pharmacokinetic interaction of cytochrome p450 3A-related compounds with rhodamine 123
, a p-glycoprotein substrate, in rats pretreated with dexamethasone.
(a cationic fluorophore) was used to monitor the mitochondrial membrane potential due to its accumulation inside the cells.
5Diphenyl tetrazolium bromide (MTT),GSH,sulforhodamine B (SRB), rhodamine 123
(Rh123), Adriamycin (ADM), taxol, LY294002, and 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) were purchased from Sigma Chemical (St.
Dimethyl sulfoxide (DMSO), Rhodamine 123
, propidium iodide (PI), and other chemicals were obtained from Sigma (St.
The C3435T mutation in the human MDR1 gene is associated with altered efflux of the P-glycoprotein substrate rhodamine 123
from CD56+ natural killer cells.
production and dihydrorhodamine 123 (DHR) is oxidised by the neutrophil reactive oxygen species (ROS) to rhodamine 123
(RHO) releasing a fluorescent green signal which can be measured by flow cytometry.
3 kDa), Rhodamine 123
, aluminium hydroxide gel, acetic acid (purity 99%), formaldehyde, sodium borohydride, sodium hydroxide, N-methyl-2-pyrrolidinone, sodium iodide, methyl iodide (Sigma, USA), Sodium carbonate Sigma ultra and phosphate buffer solution (sodium phosphate dibasic Sigma ultra, 99%) were used as procured without further purification.
Comparison of blood-film microscopy, the OptiMAL[R] dipstick, Rhodamine 123
and PCR for monitoring anti-malarial treatment.
Relationship between Excretion Clearance of Rhodamine 123
and P-Glycoprotein (Pgp) Expression Induced by Representative Pgp Inducers.
The analyses include cytometry with the ultraviolet-excited dye Hoechst 33342 by itself (analysis at two emission wavelengths), Hoechst 33342 with rhodamine 123
, or analysis of CD34 expression.
The first work on positive dyes was inspired in the early 1980s by research showing that tumor cells selectively retained a positively charged dye, Rhodamine 123
MDR mediated by ABCB1: rhodamine 123