Volume reduction of the combined methanolic extracts was done by evaporating at 40[degrees]C using a Buchi Rotavapor
The organic phase having antimicrobial property was dried on Rotavapor
(Buchi R-210) at 40AdegC.
After completion of reaction, iodine was quenched by saturated aqueous solution of Na2S2O3 and acetone was recovered through rotavapor
. In a separating funnel, product was collected in organic layer by solvent extraction with ethyl acetate (3x10 ml).
El contenido de alcaloides fue concentrado, eliminando el solvente mediante un rotavapor
(HiTech, RE-52), con una temperatura de 40 [degrees]C y presion controlada.
Oil was obtained after evaporating solvent with a Rotavapor
at 65[degrees] C and then measured.
After extraction, a Rotavapor
rotary evaporator was used to remove solvent residues in each sample, with flasks kept at 100[degrees]C for 30 min.
The solvent in the collected fractions of fomentariol was evaporated to dry using Rotavapor
R-114 (Buchi, Flawil, Switzerland), and the dry sample was dissolved in methanol-d4 (99.9%).
The extracts were then dried over anhydrous sodium sulphate and concentrated to about 5 mL in vacuo (BUCHI Rotavapor
R-200 rotary evaporator).
En cada impregnacion, la pasta humeda se seco primero en un rotavapor
a 80 [grados]C y luego en una estufa a 120 [grados]C por 12 h.
Tools such as a Hettich centrifuge, Buchi rotavapor
, chamber vacuum machine and immersion circulator bath enable the duo "to push the bounds of flavor and aroma." Every drink uses something from the lab, from a sous-vide infusion to a bespoke aromatic essence.