In this assay, a sandwich complex
was formed using the beads and nanovesicles.
In the Elecsys intact PTH assay, a biotinylated anti-PTH monoclonal antibody and a ruthenium-labeled anti-PTH monoclonal antibody form a sandwich complex
with PTH, after which streptavidin-coated microparticles are added to magnetically separate out the sandwich complex
via biotin and streptavidin interaction.
The sample is incubated for 21 min at 37[degrees]C with both antibodies simultaneously, leading to the formation of a soluble sandwich complex
in the presence of ACTH molecules.
The immunological reaction consists of a first incubation where 50 [micro]L of sample, the biotinylated antibody, and the Ru-labeled antibody react for 9 min, when antibodies capture the TSH present in the sample to form a sandwich complex
. In a second step, streptavidin-coated paramagnetic microparticles are added, and during a second 9-min incubation time the biotinylated antibody attaches to the streptavidin-coated surface of the microparticles.