The water layer (bottom layer of the separatory
funnel) was removed and the organic layer was further dried using anhydrous Ca[Cl.sub.2] granular.
Thereafter, the hydrolate was subjected to liquid-liquid partition in a separatory
funnel and three washes with three 10 mL portions of dichloromethane.
The resultant mixture was centrifuged at a speed of 2,500 rpm for three minutes, and the supernatant was directly decanted into a 1 liter capacity separatory
PWW was then dissolved in 600 ml methanol and transferred to separatory
funnel (2L) and 600 ml of n-hexane was added to the mixture.
The reaction mixture was transferred to a separatory
funnel and left overnight.
The extract obtained was vacuum filtered and transferred to a separatory
funnel containing 40mL of petroleum ether.
. Problemy proektirovanija [Magnetic separators.
In a laboratory room, adults were placed into a 500 mL separatory
funnel using the aspirator.
This was acidified with HCl (pH 1.0) and the partition performed with 300ml of ethyl acetate using separatory
The mixture was poured into saturated N[H.sub.4]Cl solution (1000 mL) with a separatory
Next, 100 mL of petroleum ether was added to the suspension in a separatory
The solution was transferred to a separatory
funnel, and it was shaken with 10 mL chloroform for 2 minutes and then allowed to stand for clear separation of the two phases.