Brassicaceous plants are characterized by a pronounced metabolic flux toward sinapate, produced by the shikimate/ phenylpropanoid pathway, which is converted into a broad spectrum of O-ester conjugates.
Strack, "Sinapate esters in brassicaceous plants: biochemistry, molecular biology, evolution and metabolic engineering," Planta, vol.
Moreover, three ester derivatives of sinapic acid, methyl sinapate, [beta]-D-(3,4-disinapoyl)fructofuranosyl-[alpha]-D-(6- sinapoyl)glucopyranoside, and 1,2-disinapoyl-[beta]-D-glucopyranoside, have also shown comparable [sup.*]OH scavenging activity .
Similarly, [Cu.sup.+2]-mediated peroxidation of human LDL and peroxyl radical can attack on erythrocyte membranes resulting in AAPH-(2,2'-azobis(2- amidinopropane) dihydrochloride-) induced hemolysis; however, ethyl sinapate at a concentration of 10 [micro]M was found to act more effectively (76%) and suppressed the LDL oxidation than sinapic acid (59%).
In another study, sinapic acid and its alkyl esters were evaluated for anti-inflammatory activity in carrageenan-induced rat paw oedema model and an excellent anti-inflammatory activity of isopentyl sinapate was reported in comparison to other esters .
Kim et al., "Sinapate dehydrodimers and sinapate-ferulate heterodimers in cereal dietary fiber," Journal of Agricultural and Food Chemistry, vol.
Tests have shown that plants produce sinapate
esters and send them to the outer layer of their leaves to protect themselves.
In flowers and seeds, F5H is required for the production of sinapate esters, which are intermediates of syringyl lignin biosynthesis in higher plants, and some of them serve as precursors for important secondary metabolites .
Chapple, "Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis," Plant Physiology, vol.
In particular, the invention relates to the inhibition of the enzymatic activity of a UDP-glucose: sinapate
glucosyltransferase (SGT) in transgenic plant cells.