Southern blot


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Related to Southern blot: Northern blot

South′ern blot′



n.
a test for the presence of a specific DNA segment in a sample by observing whether single strands of DNA from the sample will bond with labeled strands from a known segment of the DNA in question.
[after Edwin M. Southern, originator of the test]
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.
References in periodicals archive ?
To date, molecular diagnoses for FSHD are generated using outdated Southern Blot techniques, which are imprecise, labor intensive and involve radioactive labeling methods which are being phased out of laboratory use.
To determine the stable integration of the OsCTZFP8 gene, Southern blot analyses were performed with transgenic lines.
The conditions of PCR amplification was performed according to Lee et al.(2012a).Integration of the MsHsp23gene into the alfalfa plant genomes was further confirmed with Southern blot analysis.
Southern blot hybridization was performed using DIG High Prime DNA Labeling and Detection Kit 2 (Roche Molecular Systems, Pleasanton, CA, USA).
Southern blot analysis was performed to determine whether pFJ1, pFJ4, and pFJ6 integrated into the J7-F chromosome.
RNase A (10[micro]g/well) was added to the lysed cells and genomic DNA was isolated from each cell line using the PI-1200 DNA isolator (Kurabo, Osaka, Japan) for Southern blot analysis.
To corroborate the position of inserts in the restriction fragments, Southern blot hybridization was fulfilled using pMC505 as probe (data not shown).
The RFLPs could then be used within affected families to trace the X chromosome carrying the mutant gene using the Southern blot technology.
Common mtDNA mutation was detected by multiplex PCR and telomere shortening was tested by Southern blot analysis.
The study further used southern blot analysis and immunofluorescence to analyse the alternative lengthening of telomeres mechanism.
The researchers compared the characteristics of seven GFP-modified strains to an unmodified "parent" isolate, and they confirmed the presence of the protein by using a molecular test method called "Southern blot analysis" and by direct observation with a confocal laser scanning microscope.

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