Subsequently, to obtain single-copy insertion transgenic lines, Southern blot analysis
The conditions of PCR amplification was performed according to Lee et al.(2012a).Integration of the MsHsp23gene into the alfalfa plant genomes was further confirmed with Southern blot analysis
. Twenty micrograms of genomic DNA was digested with EcoR1 and separated electrophoretically on a 1.0%agarose gel.
The Southern blot analysis
shows the presence of the DNA forms that are typical of geminivirus replication in symptomatic plants (Fig.
Arrows, polymerase chain reaction primers to detect homologous recombination; Bar, probe for Southern blot analysis
Southern Blot Analysis
. Southern blot analysis
was performed to determine whether the shuttle vectors (pFJ1, pFJ4, and pFJ6) had integrated into the J7-F chromosome and to measure their copy numbers.
DNA Extraction, PCR Detection, and Southern Blot Analysis
. Genomic DNA of rice plants was extracted from leaf tissue using CTAB protocol according to .
RNase A (10[micro]g/well) was added to the lysed cells and genomic DNA was isolated from each cell line using the PI-1200 DNA isolator (Kurabo, Osaka, Japan) for Southern blot analysis
. Recombinant ES cell clones were identified by Southern blot analysis
using a 5'-probe on EcoRV-digested genomic DNA for the first screening, and a 3'-probe on Kpnl, EcoRI, or Spel-digested genomic DNA and a 5'-probe on BstEII-digested genomic DNA for the second screening (sequence of each probe is available in Supporting information).
Abnormal methylation can be determined by Southern blot analysis
, methylation-specific PCR, or methylation-specific multiplex ligation-dependent probe amplification.
Common mtDNA mutation was detected by multiplex PCR and telomere shortening was tested by Southern blot analysis
The study further used southern blot analysis
and immunofluorescence to analyse the alternative lengthening of telomeres mechanism.
The researchers compared the characteristics of seven GFP-modified strains to an unmodified "parent" isolate, and they confirmed the presence of the protein by using a molecular test method called "Southern blot analysis
" and by direct observation with a confocal laser scanning microscope.
S1 nuclease pulsed-field gel electrophoresis and Southern blot analysis
were used to identify the sizes of [bla.sub.NDM-1]-carrying plasmids.