Keywords: aprE gene, Bacillus subtilis, Subtilisins
Results: The results demonstrated that aprE gene would not be able to produce further active subtilisin E.
Like the subtilisins, the trypsins are inducible by environmental cues [27, 28].
solani isolate from tomato in medium, containing heat-stable potato proteins, due to the presence among these proteins of a large amount trypsin and subtilisin inhibitors [34, 35].
These different subtilisins may play different roles in pathogenicity increasing the scale of adaptability to the host, or having different survival functions in several habitats under extreme ecological conditions (BAGGA et al., 2004).
Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae.
* withdrawal of 16 OESs: clichlorvos, divinylbenzene (mixed isomers), furfuryl alcohol (FA), glycerol trinitrate, nitrogen dioxide, nitrogen monoxide, sulphur dioxide, propylene dinitrate, ethylene dinitrate, subtilisins, sulphuric acid and five glycidyl ethers (2,3-epoxpropyl ethers).
HSE has published chemical hazard alert notices (CHANs) for nine of the 16 withdrawn OESs to help employers control exposure: divinylbenzene (mixed isomers), furfuryl alcohol (FA), glycerol trinitrote, nitrogen dioxide, nitrogen monoxide, sulphur dioxide, ethylene dinitrate, subtilisins and sulphuric acid.
The subtilisin protease family Pr1 is the main enzyme produced by entomopathogenic fungi during the infection process, although the trypsin- like protease Pr2 is the first to appear during in vitro growth on the cuticle (Gillespie et al., 1998).
anisopliae subtilisin and trypsin-like activities are referred to as Pr1 and Pr2, respectively.
They replicated DNA with the same technique used by Gold, Riordan, Beaudry, and Joyce, but this time they chose a specific piece that contained the code for making an enzyme called subtilisin. In essence, they were working with variations on a gene.
In nature, subtilisin breaks apart the chains of amino acids that make up certain proteins.