Briefly, in the first step, the precursor, poly(succinimide
) (PSI), was prepared by thermal polycondensation in mesitylene/sulfolane (Fluka Chemical provenience) of L-aspartic acid (from Fluka Chemical), at 180[degrees]C, for 6 h, in the presence of o-phosphoric acid (analytical reagent, 85%, from Chemical Company) as catalyst.
(79) outlined the synthesis and evaluation of a series of succinimide
hydroxamic acids, which were prepared and evaluated for HDAC inhibition and antiproliferation.
formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins.
The pyrene group of PBASE bonds to the six-membered ring structure on the surface of graphene by the [pi]-[pi] stacking force, and the succinimide
can covalently bond with the amino group on the probe DNA.
After washing with distilled water, the surface was activated by a mixture of EDCxHCl (0.52 M) and N-hydroxy succinimide
(0.87 M) for 30 min, washed with distilled water, and then treated with DR5 (100 [micro]g/mL, 4 [micro]L) at room temperature for 60 min.
Following chemical modifications, the surfaces of FDS-NPs were further cross-linked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxy succinimide
(NHS) system, followed by glutaraldehyde cross-linking and bioconjugation with specific monoclonal antibody (0.1 mg/ml, ab33023; Abcam, UK) towards C.
The working solution was prepared by adding 0.6 mg (3 [micro]mol) N-ethyl-N-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) and 1.2 mg (10 [micro]mol) N- hydroxyl Succinimide
(NHS) in 125 [micro]l 0.5 M2-(N-morpholino) ethanesulfonic acid (MES) buffer according to the Gruttner et al (17).
FCT paints typically use succinimide
and 1,2,4-triazole as part of the formulation for the sake of balancing cure speed, open time, and pot life.
However, they added that citrinin in combination with NDPS N-(3,5-dichlorophenyl) succinimide
(NDPS) and N-nitrosodimethylamine (DMN) can induce kidney tumor in rats, which was renal cell tumor (adenoma).
The mixture was allowed to cool down to room temperature and regenerated succinimide
was filtered off.