Analyzer: Cell Analysis by High Content Cell Analyzer (HCCA), (Nexcelom, Bioscience in Lawrence, MA, USA).
Once the HIV virus enters the body, it attaches itself to a white blood cell called CD-4 or alternatively called T4 cell
. They are the chief fighters of disease in the body.
The Cellometer Model Auto T4 cell
counter automates cell counting, a traditionally manual process that is time-consuming, tedious, and susceptible to operator judgment.
By the way, ask your local AIDS service organization for more information about viral loads tests and the T4 cell
"At least two studies have found that echinacea lowers T4 cell
levels," says Jack Gwaltney.
Greene, a professor of medicine at the University of California, San Francisco, explained in the September 1993 Scientific American, researchers are increasingly abandoning the direct cell-killing theory because HIV does not infect enough cells: "Even in patients in the late stages of HIV infection with very low blood T4 cell
counts, the proportion of those cells that are producing HIV is tiny--about one in 40.
New entries include MRI (magnetic resonance imaging), attention deficit disorder, surgicenter, T4 cell
, and blunt trauma.
The technique has been especially helpful in AIDS and ARC cases, particularly in following patients with T4 cell
counts less than 475/mm (sup 3)and T4/T8 ratios less than 0.75.
That scientific consensus is likely to pressure FDA to put more weight on a drug's ability to boost T4 cell
levels in evaluating AIDS drugs in the future.
The T4 Cell
Analyzer: Cell Analysis by High Content Cell Analyzer (HCCA); Nexcelom, Bioscience in Lawrence, MA, USA: Once cell growth became maximal at confluence, cells were exposed to each organic chemical mixture at 2,000 [micro]g/mL concentration for 48 hours in an incubator at 37[degrees]C and 5% C[O.sub.2].
The AIDS virus is known for its marked preference for a particular immune system cell, the T4 cell
, which orchestrates the immune response.
To confirm their tumorigenicity, we injected 3 x [10.sup.6] T1 and T4 cells
in 100 [micro]L of PBS into Balb/c nude mice and monitored tumor formation as described above.