/ novobiocin soup muller-kaufman (mkttn) (according to iso 6579)
For selective enrichment, aliquots were transferred to test tubes containing Tetrathionate
(Oxoid) and Rappaport Vassiliadis (Oxoid) broths, and the respective tubes incubated at 37[degrees]C for 24h and at 42[degrees]C for 24h.
detection, sample homogenates were enriched using tetrathionate
broth and incubated at 35[degrees]C for 24 h before being plated on xylose lysine tergitol-4 agar at 35[degrees]C for 24 h before detection.
Standard bacteriological procedures were performed for bacteria management: samples were transported in 10 ml of Buffered Triptone-Water incubated for 24 h at 37[degrees]C, pre-enriched in Rappaport Vassiliadis Broth and Tetrathionate
Broth Base (Difco Laboratories, United States).
After that, 1 ml of the pre enrichment broth was transferred to 10 ml Tetrathionate
broth and incubated at 37[degrees]C overnight (18-24 hr).
1 ml of pre-enrichment to 10 ml of Rappaport Vassiliadis (RVS) broth(Oxoid) and 1 ml to 9 ml of tetrathionate
of sodium and bright green (MKTTn) broth (Oxoid), followed by incubating of RVS and MKTTn for 24 h at 42[degrees]C and 37[degrees]C respectively.
6 the first anodic peak was pH independent, which is likely to represent oxidation of the electrochemically formed tetrathionate
Selective enrichment was performed in tetrathionate
(TT) broth and modified Rappaport-Vassiliadis (RV) broth, followed by incubation at 36 [+ or -] 1[degrees]C for 24 h and 42[degrees]C for 24-48 h, respectively.
1 ml of the culture was added to one test tube with 10 ml of tetrathionate
broth and to another with selenite, to which were added 2 drops of lugol and 2 drops of green coloring at 0.
No Salmonella species were detected on brilliant green agar (Oxoid Ltd, Hampshire, United Kingdom), even after enrichment in tetrathionate
Formulation Original JP EU BP USP <62> <2022> USP Harmonized USP Gum Stems 1865 and branches Mossel* X X X Rappaport X X X (RSV)** Selenite' X X X Cysteine Tetrathionate
X X X X X * * Contains selective inhibitory agent.