Briefly, fecal samples were incubated in tetrathionate
broth (Oxoid, USA) followed by plating on Hektoen enteric agar (BD, Germany).
Each pre-enrichment broth (5ml) was transferred to 10ml of tetrathionate
broth and incubated at 42[degrees]C for 24hrs.
At the same time 1 mL homogenate was transferred in to 10 mL Tetrathionate
Broth (Merck 1.05285) and then incubated for 24 hours at 43[degrees]C.
After pre-enrichment, faecal samples were selectively enriched in tetrathionate
broth (TTB) at 42AdegC for 24 h for salmonella.
Jarvis, "Early recovery of Salmonella from food using a 6-hour non-selective pre-enrichment and reformulation of tetrathionate
broth," Frontiers in Microbiology, vol.
pY546 contained an incomplete copper resistance operon (pcoBCDE/cusRS, ORF91-96) and several clusters of genes related to resistance to other metals, including arsenic (arsABCDR, ORF100-105), tetrathionate
(ttrABCDRS, ORF142-146) and tellurite (terCDEF, ORF153-156).
The enrichment broths used were Tetrathionate
broth (TT) for Salmonella, cefixime-tellurite trypticase Soy Broth (CT-TSB) for STEC, and peptone sorbitol bile broth (PSB) for Yersinia.
Briefly, the method consists of the following steps: (A) preenrichment (Lactose Broth, Difco/BD, USA); (B) selective enrichment (Tetrathionate
broth (TT) and Selenite Cysteine broth (SC), Oxoid, England); (C) differential plating (Hektoen Enteric agar (HE), Salmonella Shigella agar (SS) and Xylose-Lysine-Desoxycholate agar (XLD), Oxoid, England); and (D) biochemical tests (Lysine Iron Agar (LIA) and Triple Sugar Iron agar (TSI), Difco/BD, USA).
Draize, "Sodium tetrathionate
and methylene blue in cyanide and carbon monoxide poisoning," Science, vol.
After incubation, samples were spread on Mannitol Salt Agar (Oxoid), Baird-Parker Agar (Difco), Cetrimide Agar (Difco), MacConkey Agar (Difco), Eozine Methylene Blue Agar (Difco), Fluid Tetrathionate
Medium (Difco), Xylose-Lysine Deoxycholate Agar (Difco), Bismuth Sulfite Agar (Difco) and Enterococcosel Agar (Difco) to determine the presence of specific microorganisms according to pharmacopeia (USP 2009).
detection, sample homogenates were enriched using tetrathionate
broth and incubated at 35[degrees]C for 24 h before being plated on xylose lysine tergitol-4 agar at 35[degrees]C for 24 h before detection.