For instance, recently, thermolysin
enzyme intended for use in dairy products to improve taste, protein solubility and digestibility of dairy products has been recognized as GRAS.
Among the digests of the water-insoluble protein fraction prepared from muscle, a thermolysin
digest demonstrated the highest activity among those that were tested.
They contain enzymes from different origins, such as collagenases from higher organisms, hemorrhagic toxins from snake venoms, and thermolysin
(50 mg/mL; Sigma, USA) was also used to digest intestine tissues for comparison.
Fuji-type landscape: Application to prolyl endopeptidase and thermolysin
," Biopolymers, vol.
Wu, "Effect of sonication on thermolysin
hydrolysis of ovotransferrin," Food Chemistry, vol.
Yoshikawa, "Peptide inhibitors for angiotensin i-converting enzyme from thermolysin
digest of dried bonitot," Bioscience, Biotechnology, and Biochemistry, vol.
Bladder biopsies were cut into small pieces and incubated overnight at 4[degrees]C in solution containing 500 mg/mL thermolysin
Quantitation of energy transfer between tryptophan and terbium(III) or europium(III) in thermolysin
," Biochemical and Biophysical Research Communications, vol.
Wasserman, "Surface localization of zein storage proteins in starch granules from maize endosperm: proteolytic removal by thermolysin
and in vitro cross-linking of granule-associated polypeptides," Plant Physiology, vol.
Some proteases such as pepsin and pronase are able to inactivate LPO by proteolysis but chymotrypsin did it very slowly and trypsin and thermolysin
are not active against LPO .
PK was replaced by a metalloproteinase thermolysin
, which was shown to readily digest [PrP.sup.C] into small fragments while leaving [PrP.sup.Sc] intact [48, 49].