The stem tissues were cut into small pieces, taken into eppendorf tube, homogenized and digested with extraction buffer (2% cetyl trimethylammonium
bromide, 1% polyvinyl pyrrolidone, 100 mM Tris-HCl, 1.4 M NaCl, 20 mM EDTA, 3% [beta]-mercaptoethanol) at 65AdegC for 15 min.
com) according to the manufacturer's protocol or the cetyl trimethylammonium
bromide-based standard purification.
The studied monomer was 2-methacryloyl oxyethyl trimethylammonium
chloride in the presence of AIBN.
Cationic guar gum was prepared by microwave-assisted using 3-chloro-2-(hydroxypropyl) trimethylammonium
chloride as cationic etherifying agent.
Total genomic DNA was extracted from the beetle bodies and from the mycelia of fungal isolates following a modified cetyl trimethylammonium
bromide (CTAB) protocol (Doyle and Doyle 1987).
The total genomic DNA was extracted from approximately 100 mg of folioles by following the method Cetyl trimethylammonium
bromide (CTAB) described by Doyle and Doyle (1987) with modifications: increased concentration of polyvinylpyrrolidone (PVP) from 1% to 2%, CTAB from 2% to 5% and [beta]-mercaptoethanol from 0.2% to 2%, furthermore the time of incubation at 65[degrees]C was reduced from 60 min to 30 min.
This method has been used for preparation of CNFs combination with following mechanical disintegration: (2,3-epoxypropyl) trimethylammonium
chloride [89, 226, 227], (2-chloroethyl) trimethylammonium
chloride , and (2-hydrazinyl-2-oxoethyl)-trimethylazanium chloride  were commonly used as cationization reagents.
Other studies indicate that dicationic porphyrins with trimethylammonium
with the positive charges being at adjacent position was more efficient in photodynamic inactivation of E.
Kim, "A relative and absolute quantification of neutral N-linked oligosaccharides using modification with carboxymethyl trimethylammonium
hydrazide and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry," Analytical Biochemistry, vol.
1993) and n-decyl trimethylammonium
bromide (Inacio et al.
The most significant ones could be listed as a) Sodium dodecyl sulfate (SDS) Chandrasekhar et al , b) Salt and oleic acid, c) Cetyltri methyl ammonium bromide (CTAB), Jiang , d) Dodecyle trimethylammonium
bromide (DTAB) and sodiumoctanoate (SOCT), Li et al , e) Hexadecyltri methyl ammonium bromide(HCTAB),Yu et al , f) Polyvinyl pyrrolidone' 1q (PVP), Pantzali et al , and g) Gum Arabic, Madni et al .
Genomic DNA was extracted from mycelia tissues given different treatment using the Cetyl trimethylammonium
bromide (CTAB) method (Kidwell et al., 1992).