(tripalmitin) (Sigma-Aldrich, Milan, Italy) was used as a standard for lipid extraction (Holland, Gabbott 1971).
synthetized chitosan-coated NLCs made of natural lipids (glyceryl tridecanoate and glyceryl tripalmitate
) and two surfactants (soy lecithin and Kolliphor[R] HS15) aiming to prevent and reverse atherosclerotic lesion development through decreasing macrophage cholesterol content.
n-hexane and methanol were purchased from LAB-SCAN Analytical sciences (Dublin, Ireland), sodium and potassium hydroxides, sodium thiosulphate and anhydrous magnesium sulphate were procured from Merck (Darmstadt, Germany) while glyceryl tripalmitate
, glyceryl tristearate, glyceryl trioleate and glyceryl trilaurate (all E 99%) were purchased from Fluka (Steinheim, Germany).
Lipids like glyceryl tripalmitate (tripalmitin), glyceryl monostearate, glyceryl tristearate, and surfactants--poloxamers (Pluronic F68 and Pluronic F127)-were purchased from Sigma Aldrich.
The solubility of the drug was determined in six different lipids--glyceryl monostearate, glyceryl behenate (Compritol 888 ATO), glyceryl tripalmitate (tripalmitin), glyceryl palmitostearate, glyceryl distearate, and cetyl palmitate.
Glyceryl tripalmitate (tripalmitin) showed maximum drug solubilizing capacity of 120 [+ or -] 10 mg drug/gram of lipid while glyceryl palmitostearate and cetyl palmitate showed the next highest solubilizing capacity of 70 [+ or -] 10 mg drug/gram of lipid.
In turn, in tripalmitate
fed rats, no significant change of activity of this parameter was observed compared to control diet .