Western blot analysis


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Western blot analysis

n.
A technique for identifying specific proteins in a mixture, in which the proteins are separated by electrophoresis, transferred to a membrane of nitrocellulose or another material, and identified by their reaction with labeled antibodies.

[Playful coinage after Southern blot analysis.]
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.
References in periodicals archive ?
D, Expression levels of proliferation-associated proteins were evaluated by western blot analysis. E, Percentage of apoptotic cells was determined by FITC Annexin V/Dead Cell Apoptosis kit and flow cytometry.
The critical candidate gene was validated by qRT-PCR and Western blot analysis of A549 cells treated with LPS and LXA4 at different concentrations and time intervals.
Western blot analysis showing detectable levels of prion protein in the chronic wasting disease- affected cervid brain specimens used to evaluate the susceptibility of the human prion protein (PrP) to conversion by chronic wasting disease prions.
Western Blot Analysis. Tissues or cells were lysed and centrifuged.
Western blot analysis showed that PPAR[alpha] shRNA silence significantly decreased the LC3-II levels in SW480, Hela, and HEK293T cell lines (Figure 1(a)).
And the western blot analysis showed an increased expression of Beclin-1 and LC3-II and a decreased expression of p62 in the NP cells treated with high glucose concentrations when compared with the control group (Figure 2).
Lung tissues were collected for histopathological staining, immunohistochemistry assessment, Western blot analysis, and quantitative real-time PCR (qRT-PCR) examination.
We confirmed that [H.sub.2][O.sub.2]-treated His-DJ-1 showed a distinct increase in OxiDJ-1 and a gradual decrease in DJ-1 levels when DJ-1 concentration was gradually increased by Western blot analysis (Figures 1(a) and 1(b)).
Immunoprecipitation and Western Blot Analysis. Cells, treated or not with 20 ng/ml KGF for the indicated times, were lysed in RIPA buffer.
(a) Representative and statistical results of Western blot analysis showed the levels of cPKC[beta]II in the cortex, hippocampus, and striatum at 2 weeks after STZ injection.
For Western blot analysis, total protein from each lysate was loaded and separated by 10% SDSPAGE and transferred onto the nitrocellulose membrane.