This was followed by es terification reaction with aliphatic ca rbo xylic ac ids with vary ing chain lengths i.e.
Biological StudySix bacterial s trains Staphylococcus aureus,Bacillus subtilis, Micrococcus luteus, Salmonella setubal, Escherichia coli and Pseudomonas aeruginosa were us ed to check the antibacterial activity of s ome of s ynthes ized a zo xy d ies ters (1, 2,8, 9, 14, 15 and 16) f a liphatic ca rbo xylic ac ids byus ing agar we ll d iffus ion method at 1 mg/ mL (1000g mL-1) concentration.
The compounds 1, 2, 8, 9, 14, 15 and 16 (azo xy dies ters of a liphatic carbo xylic acids ) were s ubjected to check antifungal activity agains t s ix fungal s trains (Mucor species, Aspergillus niger, Aspergillus flavus, Alternaria solani, Fusarium solani and Aspergillus fumigatus) by us ing agar tubedilution method [18, 19] Res ults are pres ented in Table-3.
(c m-1) KBr :3191(OH), 1600,1495(C=C Ar), 1541,1330(-N2(O)-),850 (p-dis ubs titution); 1H-NM R (300M Hz, DMSO-d6) (pp m): 8.27(d, J =9Hz, 4H), 7.29(d, J=9Hz, 4H),3.42 (s , 2H), 13C-NM R (75 MHz, DMSO-d6) (pp m):155.5, 145.2, 125.2, 122.4.The aliphatic carbo xylic ac ids were firs t converted to their acid chlorides follo wing the reported procedure .
Agar we ll d iffus ion method was us ed to check the antibacterial activ ity of 1, 2, 8 , 9, 14, 15 and 16 among the s ynthes ized azo xy d ies ters of aliphatic ca rbo xylic ac ids agains t s ix bacterial s trains ; three gra m pos itive Staphylococcus aureus (ATCC6538), Bacillus subtilis (ATCC 6633) and Micrococcus luteus (ATCC 10240) and three gram negative Salmonella setubal (ATCC 19196), Escherichia coli (ATCC 15224) and Pseudomonas aeruginosa (ATCC 15692) [18, 19].