Detection of grain infestation caused by the granary weevil (Sitophilus granarius L.) using zymography
for [alpha]-amylase activity.
assay was performed to evaluate the activity of MMP-2 and MMP-9 as previously described (12).
: Gelatin-specific zymography
technique was used for determination of MMPs activities in follicular fluid and seminal plasma.
 investigated enzyme activity by zymography
of MMPs-2, among others, in the serum of healthy men (n = 20), with BPH (n = 26), with localized PCa (n = 10), and with metastatic PCa (n = 15).
Gelatin Gel Zymography
. The supernatant of the cocultured microencapsulated cells was centrifuged at 2000 rpm for 10 min; next, it was frozen in -80[degrees]C.
Noteworthy, we analyzed MMP-9 levels using an ELISA technique, in contrast to many previous studies on MMP-9, which determined its levels using a zymography
. MMP-2/collagenases activity was assessed by gelatin zymography
by using conditioned medium obtained from BM-MSCs essentially as previously reported .
Li, "Reveal the response of enzyme activities to heavy metals through in situ zymography
," Ecotoxicology and Environmental Safety, vol.
MMP-9 or gelatinase-B, analysis by gelatin zymography
from these same PISF samples, revealed that any form of mMp-9 or total MMP-9 was not able to differentiate peri-implant health and disease (Figure 2(a)).
The result of the gelatin zymography
demonstrated that the activity of MMP2/9 was decreased in the knockdown group when compared with the scrambled shRNA group (Figure 6).
. In order to confirm the character of oviductal MMPs, OF was incubated in the presence of APMA (aminophenylmercuric acetate) to activate the latent zymogen and induce the transition of proMMP to MMP, and analysed by zymography
We will investigate effects on cell proliferation (DNA assay), elastic matrix deposition (Fastin assay), fiber formation and crosslinking (desmosine assay), and elastic matrix breakdown processes mediated by matrix metalloproteases (MMPs) (western blot, gel zymography