zymography


Also found in: Medical, Wikipedia.

zy·mog·ra·phy

 (zī-mŏg′rə-fē)
n.
An electrophoretic technique used to detect the identity and activity of a protease in a sample or tissue, in which the protease is allowed to react with a protein substrate, as in a gel, and the products of the reaction are identified with stains or by other means.

Mentioned in ?
References in periodicals archive ?
Detection of grain infestation caused by the granary weevil (Sitophilus granarius L.) using zymography for [alpha]-amylase activity.
Gelatin zymography assay was performed to evaluate the activity of MMP-2 and MMP-9 as previously described (12).
Zymography: Gelatin-specific zymography technique was used for determination of MMPs activities in follicular fluid and seminal plasma.
[17] investigated enzyme activity by zymography of MMPs-2, among others, in the serum of healthy men (n = 20), with BPH (n = 26), with localized PCa (n = 10), and with metastatic PCa (n = 15).
Gelatin Gel Zymography. The supernatant of the cocultured microencapsulated cells was centrifuged at 2000 rpm for 10 min; next, it was frozen in -80[degrees]C.
Noteworthy, we analyzed MMP-9 levels using an ELISA technique, in contrast to many previous studies on MMP-9, which determined its levels using a zymography technique [11].
Gelatin Zymography. MMP-2/collagenases activity was assessed by gelatin zymography by using conditioned medium obtained from BM-MSCs essentially as previously reported [17].
Li, "Reveal the response of enzyme activities to heavy metals through in situ zymography," Ecotoxicology and Environmental Safety, vol.
MMP-9 or gelatinase-B, analysis by gelatin zymography from these same PISF samples, revealed that any form of mMp-9 or total MMP-9 was not able to differentiate peri-implant health and disease (Figure 2(a)).
The result of the gelatin zymography demonstrated that the activity of MMP2/9 was decreased in the knockdown group when compared with the scrambled shRNA group (Figure 6).
Gelatine Zymography. In order to confirm the character of oviductal MMPs, OF was incubated in the presence of APMA (aminophenylmercuric acetate) to activate the latent zymogen and induce the transition of proMMP to MMP, and analysed by zymography.
We will investigate effects on cell proliferation (DNA assay), elastic matrix deposition (Fastin assay), fiber formation and crosslinking (desmosine assay), and elastic matrix breakdown processes mediated by matrix metalloproteases (MMPs) (western blot, gel zymography).