Total hemocyte count, phagocytic activity and
phagocytic index, and serum antioxidant activity were determined to assess innate immune system function and oxidative stress, respectively.
Macrophage engulfment percentage and
phagocytic index were evaluated by the formulas (Tatarczuch et al.
a) Phagocytic rate ([R.sub.P]), b)
Phagocytic index ([I.sub.P]).
The
phagocytic index was calculated as the percentage of cells performing phagocytosis using the mean number of particles per cells.
Additionally, we examined the effect of coculture on the
phagocytic index of M[PHI] in response to zymosan exposure.
Determination of phagocytic activity and
phagocytic index Phagocytic activity was determined according to Kawahara et al.
Phagocytic index (a) showed significantly higher response in all three polysaccharides administered groups.
The results were expressed as percentage of heterophils with bacteria and
phagocytic index (PI), where PI = (the percentage of heterophils containing bacteria) x (the average number of bacteria per ingesting heterophil).