The chromatin structure of the spermatozoa was evaluated using acridine
orange according to a methodology proposed by Bochenek et al.
Phase contrast, fluorescence microscopy as well as flow cytometry using Hoechst 33342, acridine
orange/ethidium bromide and Annexin V-F1TC as cellular probes were used to evaluate the induction of apoptosis by rosamultic acid.
bryoniae leafminers, for behavioral reasons, were found to be more sensitive than males to hematoporphyrin dimethyl ether and other photosensitizers like acridine
orange, aminolevulinic acid and methylene blue (Luksiene et al.
We spiked 35-70 cells fluorescently prelabeled with Acridine
orange (DYNAL) into 0.
Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine
orange fluorescence study.
Blood was smeared on an acridine
orange-coated slide (Molecular Probes, USA), covered with a cover slip, and analyzed according to Hayashi et al.
Objective: The aim of this study was to determine whether Acridine
Orange (AO) can be used as a routine procedure in a physician's office to detect sperm deoxyribonucleic acid (DNA) fragmentation in patients with varicoceles.
And apoptosis and DNA integrity evaluated by staining the help of the acridine
It is enabled by the linear expansion of the huffy coat by the presence of a close fitting cylindrical insert or float inside a dried acridine
orange-coated capillary tube.
The slides were stained with 40 [micro]L of acridine
orange (5[micro]g/mL) and examined with a fluorescence microscope equipped with an excitation filter of 450-490 nm, using a magnification of 10x and 40x.
It is, therefore, possible that acridine
compounds, either modified or used in combination with other drugs can be used to treat the leishmaniases.
A single-cell suspension was generated by mechanical dissociation, passed through a cellulose column, spread onto a slide, fixed in 25[degrees]C methanol for 10 min, and stained with acridine
orange (Fisher Scientific, Hanover Park, IL) at a concentration of 20 [micro]g/mL in 19 mM sodium phosphate (Na[H.