Different techniques have been used to determine the integrity of DNA in which acridine
orange (AO) assay is easy and less costly.
The blood is taken in a QBC capillary tube, which is coated with Acridine
Orange [a fluorescent dye] and centrifuged.
orange/ethidium bromide double fluorescence staining
Ethyl acetate extract was subjected to antimutagenic testing through Peripheral Blood Micronucleus Assay using Swiss albino mice of different sexes aging 7 to 12 weeks and acridine
orange as staining agent [13, 14].
To increase the sensitivity, we restained the preparation with acridine
orange staining method detects inhibition ability of Q7G against influenza A/PR/8/34 virus-induced acidic vesicular organelles (AVO) formation as explained earlier.
Methods: The B16F10 cell line was treated with different concentrations of saponin (O, 4, 8, 12, 16, 20 [micro]g/ml), dacarbazine (O, 1200, 1400, 1600, 1800, 2000 [micro]/ml) and coadministration of saponin-dacarbazine (1200 da+8 sp, 1200 da+4 sp) for 24 and 48 hr and the cytotoxic effect was examined by MTT, DAPI, acridine
orange/propodium iodide, flow cytometry and caspase colorimetric assay.
4% spermatozoa DNA damage by acridine
orange staining technique that was 8-9% higher compared to this study.
Comparison of acridine
orange and Giemsa stains for malaria diagnosis.
Slides are stained in acridine
orange (AO) solution, rinsed, and then scored using a fluorescent microscope.
Coverslips were rinsed with PBS and were stained with acridine
orange for 10 min.
Chromatin condensation in cat spermatozoa during epididymal transit as studied by aniline blue and acridine