Finally, an in vivo animal model showed that adoptively
transferred cytotoxic T cells persisted in mice following administration of CD45 targeted lymphodepletion and were able to control tumor cells compared to untreated mice.
We randomly assigned the stimulus conditions, and adoptively
changed the imposed gap duration of the adjustable stimulus, based on the participants' response.
Similarly, immunodeficient mice adoptively
transferred with [CD4.sup.+]IL-[21.sup.-/-] T cells develop less severe colitis [60, 61], and mice treated in a prophylactic setup with a neutralizing IL-21R/Fc fusion protein or anti-IL-21 mAb are protected from both DSS and TNBS-induced colitis [31, 58, 59].
Bollard et al., "Enhancing the in vivo expansion of adoptively
transferred EBV- Specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients," Blood, vol.
To further confirm the role of Id1 in BMP6-induced BMMSC immunosuppressive function impairment, we adoptively
transferred OT-II T cells plus BMMSCs treated with or without BMP6 or Id1 siRNA to CD45.1 transgenic C57BL/6 mice.
To elucidate whether [Gr-1.sup.+] myeloid cells are key components in the protection against and/or resolution of fibrosis, purified [Gr-1.sup.+] myeloid cells from lungs of Clod Lipo-treated and bleomycin-challenged mice at day 5 were adoptively
transferred via oropharyngeal instillation into the lungs of other groups of mice at either day 5 or 14 after bleomycin.
In addition, lipopolysaccharide-activated dendritic cell-induced [CD4.sup.+][CD25.sup.+][Foxp3.sup.+] Tregs inhibit [CD4.sup.+][CD25.sup.-] effector T cells, and when adoptively
transferred, these Tregs suppress EAU .
Inhibition of pro-inflammatory cytokine generation by CTLA4-Ig in the skin and colon of mice adoptively
transplanted with CD45RBhi CD4+ T cells correlates with suppression of psoriasis and colitis.
Further, lung DCs in vitro have the option to imprint the expression of gut-homing integrin [alpha]4[beta]7 and CCR9 (lung-homing integrin is CCR4 ) on cocultured T cells in vitro and on adoptively
transferred cells in vivo, which guides their migration to the GI tract .
Moreover, HBV immunity can be adoptively
transferred in allogeneic bone marrow transplants.
Indeed, protocols have been developed to adoptively
transfer CMV-specific CD8+ T cells to prevent viral reactivation in transplant recipients .
The challenge of this therapy is that the adoptively
mature [CD4.sup.+] T cells have limited replicative potential.