The sensors are segmented into Cameleon, Chloride (clomeleon), Aequorin
, membrane gated (mermaid) and GCaMP.
A high-throughput assay for connexin 43 (Cx43, GJA1) gap junctions using codon-optimized aequorin
. Assay Drug Dev Technol 2013;11:93-100.
(8) Arabidopsis thaliana (thale cress) is a popular model organism in plant biology and genetics carrying the aequorin
gene, a gene producing the calcium-dependent bioluminescent protein aequorin
to measure increased [Ca.sup.2+] in response to low level 50 Hz sinusoidal EMF exposure.
Alvarez, "Mitochondrial free [Ca2+] dynamics measured with a novel low-Ca2+ affinity aequorin
probe," The Biochemical Journal, vol.
(1962) Extraction, purification and properties of aequorin
, a bioluminescent protein from the luminous hydromedusan, Aequorea.
Kraus et al., "Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin
luminescence," Journal of Receptor and Signal Transduction Research, vol.
Johnson, "Peroxidized coelenterazine, the active group in the photoprotein aequorin
," Proceedings of the National Academy of Sciences of the United States of America, vol.
CANARY uses genetically engineered B cells to produce aequorin
, a calcium-sensitive bioluminescent protein.
Among the topics are understanding the pathway to successful drug discovery, homology modeling, using aequorin
for receptor hit identification and compound profiling, yeast assays, applying fluorescence resonance energy transfer techniques to establish ligand-receptor orientation, and the immuno-precipitation and phosphorylation of the receptors.
In the 1960s and 1970s GFP along with the separate luminescent protein Aequorin
, was purified from Aequorea Victoria and was characterized by Osamu Shimomura (1, 2).
The photoprotein aequorin
was used as a bioluminescent reporter for the detection of only 1 mutation of the HBB gene using the oligonucleotide ligation reaction (28).
Euroscreen Products will transfer to PerkinElmer its GPCR screening tools and exclusive worldwide license to aequorin
technology, granted by the University of Georgia Research Foundation.