And chewing a conker - or its shell - can release a potentially fatal toxin called aesculin
which is found in all parts of the horse chestnut tree.
An aliquot of the broth was plated onto blood agar plates and incubated in a C[O.sub.2] incubator at 35[degrees]C overnight, and a bile aesculin
agar was inoculated.
Bu konuda yapilan calismalarda at kestanesi ozunde yer alan aescinin suda cozunurluk ve erime noktasina gore birbirinden ayirt edilen alfa ve beta olmak uzere 2 form triterpene saponin karisimindan olustugu; bunun disindaki bilesenlerin ise bioflavonoidler (quercetinve kaempferol),proanthocyanidin A2 (bir antioksidan) ve coumarinler (fraxin ve aesculin
) oldugu gosterilmistir.
colonies were confirmed in kanamycin aesculin
azide agar (Liofilchem, Roseto degli Abruzzi, Italy), incubated at 44[degrees]C for 4 h.
Zhang, "Micelle-mediated extraction and cloud point preconcentration for the analysis of aesculin
and aesculetin in Cortex fraxini by HPLC," Journal of Pharmaceutical and Biomedical Analysis, vol.
Arginine dihydrolase agar and Aesculin
Azide agar (Merck, Germany) were employed to perform the hydrolysis tests.
The isolates were examined for gram reaction, catalase, oxidase, ultraviolet fluorescence, argenin dihydrolase, levan production, gelatin liquefaction, aesculin
hydrolysis, hypersensitive reaction on tobacco leaves and pectolytic capability (Schaad et al., 2001).
Samples were plated onto Sheep blood agar (5%>), MacConkey agar and Bile Aesculin
Catalase-negative organisms were tested on both bile aesculin
plates (Diagnostic Media Products, SA) and with the PYR 50 test (Remel, USA) to differentiate between Streptococci and Enterococci.
For secondary identification of the isolates to the species level, different biochemical tests (oxidation fermentation test, Mannitol and salicin sugar fermentation, aesculin
hydrolysis, and CAMP test) were done based on the genus ofbacteria to be identified .
Some of the coumarins, such as aesculetin, aesculin
, and fraxin, also possess antioxidant activity.
Ltd., China), Kanamycin Aesculin
Azide Agar (Qingdao Hope Bio-Technology Co.