Flowers within a plant were assigned to the following treatments: (1) agamospermy
(flowers had their stigma removed at the beginning of anthesis); (2) autonomous self-pollination (un-manipulated bagged flowers); (3) hand self-pollination (flowers hand-pollinated with their own pollen); (4) hand cross-pollination (previously emasculated flowers hand-pollinated with pollen from another plant), and (5) natural pollination (un-manipulated and randomly selected flowers exposed to pollinators in the field).
Ten flowers in pre-anthesis of female plants were emasculated marked and bagged to verify the occurrence of agamospermy
in this species.
We performed the following five treatments: self-pollination, spontaneous self-pollination, geitonogamy, xenogamy and agamospermy
. Not all treatments co-occurred in the same individual.
With the use of this methodology we try to assess whether the plant sets fruit or not, if produce seeds without fertilization (agamospermy
, or also called apomixis) or only produce fruits by cross pollination.
Recent studies showed that the reproductive system of this species is self-compatible; it does not produce fruits via either autogamy or agamospermy
, but requires, obligatorily, pollen vectors due to buzz pollination syndrome (Andrade, 2004).
Two independent loci control agamospermy
(apomixis) in the triploid flowering plant Erigeron annuus.
Although these offspring are often outcrossed seeds, they may also be a result of agamospermy
(Crawley 1997) or, in the case of aquatic plants, widely dispersing clonal fragments (Room 1990, Philbrick and Les 1996).
Embryological investigations into the mechanism of agamospermy
in British P.