The surface profiles of the coated and uncoated glasses characterized by AFM showed that agarose
provided three-dimensional thin film on the unmodified and PLL-coated glass slides (Figures lA and 2A).
To characterize the nature of the assay interference, patient or control sera were treated with protein A-linked sepharose, streptavidin-linked agarose
, or agarose
The results of restriction enzyme digestions were examined by electrophoresis on 1% agarose
The slideshow, which should take no more than 12 minutes to present, first describes how DNA is restriction digested and separated by size using agarose
After drying, the pellet was dissolved in 20[micro]l of TE buffer and stored the DNA in-20[degrees]C for further visualization in agarose
In most of them the network is formed by biopolymers , e.g., proteins (gelatin, elastin) or polysaccharides (agarose
The topics include the two-dimensional agarose
gel electrophoresis of DNA topoisomers, single-molecule magnetic tweezer studies of type IB topoisomers, binding DNA topoisomers I and II to replication origins, assaying topoisomers II checkpoints in yeast, the cytological analysis of chromosome structural defects that result from topoisomers II dysfunction, and the depletion and mutation of topoisomerase II in animal cells.
The isolated DNA was evaluated by agarose
gel electrophoresis after staining ethidium bromide and exposure to UV light.
It was observed that the reactions employing DNA extracted with sodium hydroxide showed a weaker band than those performed with the untreated hair bulbs, upon electrophoresis of the PCR reaction on a 2% agarose
gel stained with ethidium bromide.
With allele-typing by agarose
gel and sequence mutation screening by dHPLC, we have shown that PfMSP6 allele frequencies vary over time, but intra-allele diversity is limited with few SNPs.
Noting that many researchers use ethidium bromide staining to monitor the progress of gradient separation of the DNA bands from agarose
gel slices, Spectronics said its E-Series lamps ensure a higher sensitivity of detection during such separation, without damaging the ethidium bromide-stained DNA.
Microwave-plasma was used to generate a hydrophilic surface on the (hydrophobic) polycarbonate cap so that the agarose
film would adhere to its inner surface.