Lectin Wheat germ agglutinin
(WGA) conjugated with isitiosionate of fluoresceine (WGA-FITC) has been used to identify these carbohydrates in vitro.
Apellis is currently evaluating APL-2 in clinical studies in patients with geographic atrophy (GA), in patients with paroxysmal nocturnal hemoglobinuria (PNH) who are being treated with eculizumab or who are naive to complement inhibitor treatment, in patients with warm autoimmune hemolytic anemia (wAIHA) or cold agglutinin
disease (CAD) and in patients with complement-dependent nephropathies.
Apellis Pharmaceuticals announced updated data from its Phase 2 PLAUDIT study of APL-2 in patients with autoimmune hemolytic anemia, including cold agglutinin
disease and warm antibody autoimmune hemolytic anemia.
Kumar et al that Allium cepa agglutinin
increased the level of NO, TNF-a and COX-2 in CP treated immunosuppressed rats.
In the case presented by the authors, IgM type autoantibodies lead to agglutination and hemolysis of erythrocytes by adhering to erythrocyte surface with complement (C3b) opsonization at low temperature (3-4 [degrees]C) in cold antibody AIHA (cold agglutinin
disease) which the authors were focused on and which frequently occurs secondary to infections (1, 2, 4, 5).
BioveratiVs pipeline includes a Phase III program for cold agglutinin
disease, and early stage research programs and collaborations in hemophilia, and other rare blood disorders, including sickle cell disease and beta thalassemia.
The first case report of autoimmune hemolytic anemia in brucellosis was described due to cold agglutinin
antibodies , but hemolysis was mild in their case which was resolved with antibiotic treatment for brucellosis.
Slices were then rinsed in PBS 3x for 5min each, 50% alcohol for 30min, and PBS 3x for 5 min each and incubated and gently rocked in 1 : 500 fluorescein-conjugated Wisteria floribunda agglutinin
(WFA, Vector Laboratories, Burlingame, CA) in PBS overnight (~20 hr) at 4[degrees]C.
As part of the acquisition, Bioverativ will obtain worldwide rights to True North's lead candidate, TNT009, a monoclonal antibody in development to treat cold agglutinin
disease, a hemolytic condition.
The category of cold hemolysins includes 2 distinct disease entities: cold agglutinin
syndrome and paroxysmal cold hemoglobinuria, either of which should demonstrate a DAT positive only with C3 antisera.
Serology performed to detect other infections indicated a cold agglutinin
titer of 1: 320 and an M.
Especially in endemic areas, the Widal test is still of significant diagnostic value, provided judicious interpretation of the test is made against a background of pertinent information, especially data which relate to agglutinin
levels in normal individuals and in nontyphoidal fevers common in the region .