They are also harvested for use as bait for the eel and whelk fisheries (ASMFC, 1998, 2012) and for their blood, which is used to create Limulus amebocyte
lysate (LAL) (Novitsky, 2009).
Lysate (LAL): The standard in-vitro assay used to test for endotoxin presence, much preferred over the in-vivo rabbit pyrogen test.
Endotoxin was measured using an endotoxin-specific kinetic turbidimetric in a Limulus amebocyte
lysate (LAL) assay.
The serum endotoxin was determined by the tachypleus amebocyte
lysate--azole colorimetric assay.
Ambiguities in applying traditional Limulus amebocyte
lysate tests to quantify endotoxin in nanoparticle formulations.
Endotoxin testing is done with the Limulus Amebocyte
Lysate (LAL) assay using either a kinetic assay or a gel-clot assay (AAMI, 2014a).
Circulating endotoxin was measured using the Limulus amebocyte
lysate QCL-1000 assay (Lonza, Walkersville, USA) according to manufacturer's instructions, using a previously described method .
Endotoxin evaluation of eleven lipopolysaccharides by whole blood assay does not always correlate with Limulus amebocyte
The limulus amebocyte
lysate (LAL) and CSE substrate as positive control are placed within prepackaged cartridges that could be directly read by the instrument at the wavelength of 395 nm, which optimizes the detection of the signal generated by the substrate chromogenic LAL.
In addition, the bacterial endotoxins in PLCP and AcPLCPs were determined using tachypleus amebocyte
lysate test kit (Chinese Horseshoe Crab Reagent Manufactory, Co., Fujian Province, China).