Phenacetin, paracetamol, metacetamol, oleanolic acid, tolbutamide, chlorpropamide, furafylline, dextromethorphan, 6[beta]-hydroxytestosterone, glucose 6phosphate (G6P), glucose 6-phosphate dehydrogenase (G6PDH), [beta]-nicotinamide adenine dinucleotide phosphate (NADP), and tris (hydroxymethyl) aminomethane
hydrochloride (Tris-HCl) were purchased from Sigma Chemical Co.
Phosvitin standard, acrylamide, N,N'-methylene-bis-acrylamide, tris (hydroxymethyl) aminomethane
(Tris), sodium dodecyl sulfate (SDS), ammonium persulfate, N,N,N',N'-tetramethyleethylenediamine (TEMED), glycine, glycerin, Coomassie Brilliant Blue G-250, aluminum nitrate, and Tween 20 were obtained from Sigma-Aldrich (St.
2] and 4mL NaOH (phosphatase activity) or 4 mL Tris (hydroxymethyl) aminomethane
(THAM, pH 12) for [beta]-glucosidase and centrifugation at 3500rpm for 3 min.
As compatibilizers, several polyalcohols, in some cases containing an amino group (for an easier incorporation in the epoxy matrix), such as polyethylene glycol 4000 (PEG), polysorbate 80 (TWEEN), and tris(hydroxymethyl) aminomethane
(TRIS) were used, because they are expected to interact suitably with silicates and can be incorporated in the O-I matrix.
5 M sodium chloride (NaCl), 100 mM EDTA, 10 mM Tris (hydroxymethyl) aminomethane
hydrochloride, 10% dimethylsulfoxide (DMSO), and 1% Triton X-100, pH 10.